S8830

Protease Inhibitor Cocktail

SIGMA FAST, EDTA-Free, tablet, for the inhibition of serine, cysteine, aspartic and metalloproteases, for use in purification of Histidine-tagged proteins

• Synonym(s): Protease Inhibitor Cocktail
• MDL number: MFCD00677817
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• product name: SIGMAFAST^™ Protease Inhibitor Cocktail Tablets, EDTA-Free, for use in purification of Histidine-tagged proteins
• biological source: bovine lung
• form: tablet
• storage temp.: 2-8°C

Description

General description

SIGMAFAST^™ Protease Inhibitor Cocktail Tablets, EDTA-Free is a water-soluble tablet formulation designed to inhibit serine, cysteine, aspartic and metalloproteases. The tablets contain no strong metal chelators, such as EDTA, and are compatible with sample processing by immobilized metal affinity chromatography (IMAC). The tablets also contain a unique, proprietary formulation that includes a pepstatin A salt for additional protection.

Specificity

Inhibits serine, cysteine, aspartic, and metalloproteases.

Application

The broad specificity of SIGMAFAST^™ Protease Inhibitor Cocktail Tablets makes them suitable for use in the purification of histidine-tagged proteins and as a general-purpose protease inhibitor cocktail. The tablets can also be used as components of buffers for cell lysis, and have been optimized and tested for use with HIS-Select media.

Features and Benefits

EDTA-Free: Contains no strong metal chelators, such as EDTA, which might interfere with IMAC processing.

Pepstatin A: Contains a unique, proprietary formulation that includes a pepstatin A salt for additional protection not normally obtained in a tablet format.

Broad Specificity: Inhibits serine, cysteine, aspartic, and metalloproteases, providing broad protection for protein samples.

High Yield: One tablet makes 100 mL of cocktail, and is recommended for the inhibition of the proteases present in a maximum of 20 g of cell mass in 100 mL of extract.

Long Stability: The tablets are stable as supplied for at least 2 years at 2–8 °C, and the reconstituted protease inhibitor solution (1´ or 10´) is stable for at least 2 weeks at 2–8 °C.

Components

AEBSF
Bestatin
E-64
Pepstatin A
Phosphoramidon
Leupeptin
Aprotinin

Quantity

One tablet makes 100 mL of cocktail. One tablet is recommended for the inhibition of the proteases present in a maximum of 20 g of cell mass in 100 mL of extract.

Physical form

Tablet that is water soluble

Storage and Stability

The tablets are stable as supplied for at least 2 years at 2–8 °C. The reconstituted protease inhibitor solution (1´ or 10´) is stable for at least 2 weeks at 2–8 °C.

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Pictograms: GHS05
• Signal Word: Danger
• Hazard Statements: H314
• Precautionary Statements: P260 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363
• Hazard Classifications: Eye Dam. 1 - Skin Corr. 1A
• Storage Class Code: 8A - Combustible corrosive hazardous materials
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves