indirect immunofluorescence: 2-4 μg/mL using human HeLa cells fixed with paraformaldehyde-Triton western blot: 0.5-1 μg/mL using nuclear extracts of human HeLa cells
Retinoblastoma-associated binding protein 46 (RbAp46) is also termed as retinoblastoma-binding protein 7 (RBBP7) and has 89% amino acid identity with RbAp48. It belongs to the WD-repeat protein family and has four repeats that ends with Trp-Asp(WD) residues. This protein is a vital component of the histone modifying and remodeling complexes. RBBP7 is mapped to human chromosome Xp22.13
Specificity
Anti-RbAp46, C-terminal specifically recognizes human RbAp46.
Immunogen
synthetic peptide corresponding to amino acids 411-425 of human RbAp46, conjugated to KLH via an N-terminal added cysteine residue. The immunizing peptide is conserved in human and differs from the mouse sequence by one amino acid.
Application
Anti-RbAp46, C-terminal antibody produced in rabbit has been used in western blotting and immunofluorescence.
Biochem/physiol Actions
Retinoblastoma-associated binding protein 46 (RbAp46) plays an important role in modification and remodeling of chromatin during cell growth and differentiation. It plays a key role in the nucleosomal remodeling and deacetylation complex (NURD). It may also involve in histone deacetylation and transcription repression.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Neurons modulate gene expression in response to extrinsic signals to enable brain development, cognition, and learning and to process stimuli that regulate systemic physiological functions. This signal-to-gene communication is facilitated by posttranslational modifications such as S-nitrosylation, the covalent attachment of
Characterization of the gene encoding mouse retinoblastoma binding protein-7, a component of chromatin-remodeling complexes
Yang J, et al.
Genomics, 80(4), 407-415 (2002)
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