Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

R3404

Sigma-Aldrich

Reagent Alcohol

95%, Used for histology tissue preparation

Synonym(s):

Ethanol, RGA, denatured alcohol

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

CAS Number:
64-17-5
MDL number:
MFCD00003568
UNSPSC Code:
15101802
PubChem Substance ID:
329824023
NACRES:
NA.47

vapor density

1.59 (vs air)

Quality Level

100

form

liquid

expl. lim.

3.1-27.7 % (lit.)

concentration

95%

color

colorless

bp

78 °C (lit.)

mp

-114 °C (lit.)

density

0.786-0.818 g/L

application(s)

hematology
histology

storage temp.

room temp

InChI

1S/C2H6O/c1-2-3/h3H,2H2,1H3

InChI key

LFQSCWFLJHTTHZ-UHFFFAOYSA-N

General description

Reagent Alcohol is denatured ethanol. It is widely used in histology and cytology for tissue processing.

Application

Reagent Alcohol has been used for dehydrating tissue samples before embedding in histology.

Components

95 parts of specially denatured ethyl alcohol 3A with 5 parts of isopropyl alcohol. Final concentrations are ~ 85% ethanol, ~ 5% methanol and ~ 5% isopropanol. Contains 5% water.

Signal Word

Danger

Hazard Statements

H225,H319,H371

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 2

Target Organs

Eyes,Central nervous system

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

57.2 °F - closed cup

Flash Point(C)

14.0 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Evaluating Iron Flux in the Brain
Wong BX, et al.
Metals, 161-193 (2017)
Ada T Feldman et al.
Methods in molecular biology (Clifton, N.J.), 1180, 31-43 (2014-07-13)
The hematoxylin and eosin (H&E) stained tissue section is the cornerstone of anatomical pathology diagnosis. The H&E procedure stains the nucleus and cytoplasm contrasting colors to readily differentiate cellular components. However, staining results are dependent on proper specimen processing, which
U Allenstein et al.
Soft matter, 11(25), 5053-5059 (2015-06-02)
Cells require adhesion to survive, proliferate and migrate, as well as for wound healing and many other functions. The strength of contractile cell forces on an underlying surface is a highly relevant quantity to measure the affinity of cells to
Sonia Soloneski et al.
Toxicology in vitro : an international journal published in association with BIBRA, 29(5), 834-844 (2015-03-31)
In vitro effects of the carbamates pirimicarb and zineb and their formulations Aficida® (50% pirimicarb) and Azzurro® (70% zineb), respectively, were evaluated in Chinese hamster ovary (CHO-K1) cells. Whereas the cytokinesis-blocked micronucleus cytome assay was employed to test for genotoxicity
Nicholas J Niemuth et al.
Chemosphere, 135, 38-45 (2015-04-22)
The occurrence of intersex fish, where male reproductive tissues show evidence of feminization, have been found in freshwater systems around the world, indicating the potential for significant endocrine disruption across species in the ecosystem. Estrogens from birth control medications in
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service