Recognizes the human CD19 (90 kDa) integral membrane glycoprotein. 3rd Workshop: code no. 073
Immunogen
NALM-1 human cell line.
Target description
CD19 is a member of the immunoglobulin superfamily and has two Ig-like domains. The CD19 molecule is expressed on 100% of the peripheral B cells as defined by expression of κ or λ light chains. It is expressed on approximately 10% of normal human peripheral blood cells and approximately 60% of splenic lymphocytes. It is not expressed on granulocytes, monocytes or T cells as defined by CD3 expression.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
Preparation Note
Prepared by conjugation to R-phycoerythrin (PE). This orange-red dye is efficiently excited at 488 nm and emits at 578 nm.
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We have investigated the regulation mechanism of the surface sialyl-Le(X) (sLe(X)) expression level in tonsillar B cells during activation. sLe(X) antigen became strongly positive after activation, while resting B cells were weakly positive. sLe(X) structures were mainly located on O-linked
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