P6248
Anti-Parkin antibody, Mouse monoclonal
clone PRK8, purified from hybridoma cell culture
Synonym(s):
Anti-AR-JP, Anti-LPRS2, Anti-PARK2, Anti-PDJ
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About This Item
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biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
PRK8, monoclonal
form
buffered aqueous solution
species reactivity
mouse, rat, hamster, human
concentration
~2 mg/mL
technique(s)
microarray: suitable
western blot: 0.25-0.5 μg/mL using using rat brain cytosolic S1 extract
isotype
IgG2b
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... PARK2(5071)
mouse ... Park2(50873)
rat ... Park2(56816)
General description
Monoclonal Anti- Parkin (mouse IgG2b isotype) is derived from the hybridoma PRK8 produced by the fusion of mouse myeloma cells (SP2 cells) and splenocytes from BALB/c mice immunized with recombinant human Parkin. Parkin is an ubiquitin-protein ligase expresses in many tissues such as brain, testis, skeletal muscle and heart. The parkin gene encodes a protein of 465 amino acid residues with moderate similarity to ubiquitin at the amino-terminus and a ring in between ring fingers (IBR)-ring-finger motif at the carboxyl-terminus. The gene has 12 exons.
Parkin is an ubiquitin-protein ligase expresses in many tissues such as brain, testis, skeletal muscle and heart. It is a RING-type E3 ubiquitin-protein ligase that facilitates the ubiquitination of misfolded proteins and protects from neurotoxic effect caused by these proteins. Monoclonal anti-parkin antibody can be used in microarray and immunoprecipitation assays. It can also be used in western blotting. Mouse anti-parkin antibody reacts specifically with parkin (50kD approx) of rat, hamster, mouse and human.
Immunogen
recombinant human Parkin.
Application
Monoclonal anti-parkin antibody has been used in:
- Enzyme linked immunosorbent assay (ELISA)
- Immunoblotting
- Immunoprecipitation[63}
- Immunocytochemistry
- to label parkin in pull-down assay
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Koji Yamano et al.
The Journal of biological chemistry, 290(42), 25199-25211 (2015-08-12)
Damaged mitochondria are eliminated through autophagy machinery. A cytosolic E3 ubiquitin ligase Parkin, a gene product mutated in familial Parkinsonism, is essential for this pathway. Recent progress has revealed that phosphorylation of both Parkin and ubiquitin at Ser(65) by PINK1
Victoriane Peugnet et al.
Antioxidants (Basel, Switzerland), 11(4) (2022-04-24)
Heart failure, mostly associated with cardiac hypertrophy, is a major cause of illness and death. Oxidative stress causes accumulation of reactive oxygen species (ROS), leading to mitochondrial dysfunction, suggesting that mitochondria-targeted therapies could be effective in this context. The purpose
Genomic organization and expression of parkin in Drosophila melanogaster
Bae JY, et al.
Experimental & Molecular Medicine, 35(5), 393-393 (2003)
Parkin regulates kainate receptors by interacting with the GluK2 subunit
Maraschi AM, et al,
Nature Communications, 5(46) (2014)
Aaron C Pawlyk et al.
The Journal of biological chemistry, 278(48), 48120-48128 (2003-09-16)
Autosomal recessive juvenile parkinsonism is a movement disorder associated with the degeneration of dopaminergic neurons in substantia nigra pars compacta. The loss of functional parkin caused by parkin gene mutations is the most common single cause of juvenile parkinsonism. Parkin
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