The product does not need to be sequenced to get released. Unfortunately there is no in-house information about product sequence.
P6181
Endoproteinase Glu-C from Staphylococcus aureus V8
suitable for protein sequencing, lyophilized powder
Synonym(s):
V8 Protease
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50 μG
$261.00
About This Item
CAS Number:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
Recommended Products
grade
Proteomics Grade
Quality Level
form
lyophilized powder
analyte chemical class(es)
amino acids
suitability
suitable for protein sequencing
storage temp.
−20°C
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Application
Endoproteinase Glu-C from Staphylococcus aureus V8 has been used for:
- obtaining proteolytic cleavage fragments of the S-layer protein (from Bacillus stearothermophilus ATCC 12980) to perform affinity studies.[1]
- the enzymatic cleavage of native VSTx-3 (voltage sensor toxin 3) peptide for its sequence determination.[2]
- limited proteolysis of recombinant purified PimA (phosphatidylinositol mannosyltransferase).[3]
- the digestion of glycosylated hemoglobin for isotope dilution liquid chromatography-tandem mass spectrometry analysis.[4]
Biochem/physiol Actions
Endoproteinase Glu-C from Staphylococcus aureus strain V8 is a serine endoprotease, which hydrolyzes peptide bonds at the carboxyl side of glutamyl and aspartyl residues.[5] The specificity of Glu-C is dependent upon the buffer and pH employed as well as the structure around the potential cleavage site. In ammonium acetate (pH 4.0) or ammonium bicarbonate (pH 7.8), the enzyme preferentially cleaves glutamyl bonds; whereas, in phosphate buffer (pH 7.8) Glu-C will cleave at either site.[6] Glu-C is reported to be active in the presence of 0.2% SDS (sodium dodecyl sulfate) and in 4.0M urea.[7]
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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M Jarosch et al.
Microbiology (Reading, England), 146 ( Pt 2), 273-281 (2000-03-09)
The cell surface of Bacillus stearothermophilus ATCC 12980 is completely covered with an oblique S-layer lattice. To investigate sequence identities and a common structure-function relationship in S-layer proteins of different B. stearothermophilus wild-type strains, the nucleotide sequence encoding the S-layer
X. Wang et al.
Plant physiology, 111(2), 441-445 (1996-06-01)
Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) and a 66-kD protein were co-purified from solubilized microsomal preparations of the green alga Botryococcus braunii by Green A agarose, sucrose density gradient, MonoQ, and gel filtration. The 66-kD protein remained intact after 6 M urea treatment
The abalone egg vitelline envelope receptor for sperm lysin is a giant multivalent molecule.
Swanson, W.J., and Vacquier, V.D.
Proceedings of the National Academy of Sciences of the USA, 94(13), 6724-6729 (1999)
Mechanism of acylphosphatase inactivation by Woodward's reagent K.
Paoli, P., et al.
The Biochemical Journal, 855-861, 855-861 (1997)
Two tarantula venom peptides as potent and differential Na(V) channels blockers.
Cherki RS et al.
Toxicon, 77, 58-58 (2014)
Protocols
An optimized LC-MS/MS based workflow for low artifact tryptic digestion and peptide mapping of monoclonal antibody, adalimumab (Humira) using filter assisted sample preparation (FASP).
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Hi, I was just wondering you can give me the amino acid sequence for this protein. I can't find it on the page.
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