P3250
Papain from papaya latex
lyophilized powder, 0.5-2.0 units/mg solid
Synonym(s):
Papainase
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About This Item
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form
lyophilized powder
specific activity
0.5-2.0 units/mg solid
mol wt
23.4 kDa
storage temp.
2-8°C
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Application
Papain papaya latex, product P3250, has been used to hydrolyze chitosan to determine whether chitosan can be used in food preservation[1]. It is used in dissecting solutions[2]. It is used to produce Fab fragments of antibodies.[3] It is used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues.[4][5]
Used to produce Fab fragments of antibodies. Also used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues.
Biochem/physiol Actions
A cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine.
pH optimum 6.0-7.0
Also hydrolyzes esters and amides.
pH optimum 6.0-7.0
Also hydrolyzes esters and amides.
Papain papaya latex has antifungal activity against C. albicans[1]. It is a cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine.
. The pH optimum is 6.0-7.0
. Papain hydrolyzes esters and amides.
. The pH optimum is 6.0-7.0
. Papain hydrolyzes esters and amides.
Unit Definition
One unit will hydrolyze 1.0 micromole of N-alpha-benzoyl-L-arginine ethyl ester (BAEE) per minute at pH 6.2 at 25 deg C
Other Notes
View more information on papain at www.sigma-aldrich.com/enzymeexplorer.
inhibitor
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Description
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substrate
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Description
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Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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