P0393
Protein Kinase Inhibitor from porcine heart
1-3 inhibitor U/μg protein, lyophilized powder
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About This Item
MDL number:
UNSPSC Code:
12352202
Recommended Products
biological source
Porcine heart
form
lyophilized powder
specific activity
1-3 inhibitor U/μg protein
composition
Protein, ~50% biuret
storage temp.
2-8°C
Biochem/physiol Actions
These proteins bind to the catalytic subunit of cyclic-AMP dependent protein kinase, displacing the regulatory subunit and inhibiting phosphorylating activity. The cyclic-AMP binding capacity of the regulatory subunit is enhanced. Many other proteins exhibit this enhancement ability, too (Brostrom, C.O. and Kon, C., Anal. Biochem., 58, 459 [1974]).
Packaging
Package size based on protein content.
Unit Definition
One unit will transfer 1.0 picomole (10-12 mol) of phosphate from γ-32P-ATP to hydrolyzed and partially dephosphorylated casein (Product No. C 4765) per min at pH 6.5 at 30 °C.
Physical form
Supplied as lyophilized powder containing 30% glycerophosphate and 20% EDTA.
Preparation Note
Fractionated essentially by procedure of Walsh, D.A., et al., J. Biol. Chem., 246, 1977 (1971).
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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