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Sigma-Aldrich

GenElute™ HP Endotoxin-Free Plasmid Megaprep Kit

greener alternative

1 kit sufficient for 5 preparations

Synonym(s):

Gen Elute, GenElute Endotoxin-Free Plasmid Kit

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About This Item

UNSPSC Code:
41105501
NACRES:
NA.52

usage

 kit sufficient for 5 preparations

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

greener alternative category

, Aligned

storage temp.

15-25°C

Related Categories

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
The GenElute HP Endotoxin-Free Plasmid Megaprep Kit offers a simple, rapid, and cost effective method for isolating endotoxin-free plasmid DNA from recombinant E. coli cultures. Up to 5 mg of high copy plasmid DNA with ≤ 0.1 endotoxin units/μg can be recovered from 200 mL - 1.0 L LB medium or 200 - 600 mL TB medium of E. coli culture in less than 1.5 hours. Note that the actual yield depends on the strain, the plasmid, and the culture medium used.

High-quality, endotoxin-free DNA is ready for immediate use in downstream applications such as restriction digest, ligation, sequencing, PCR, transformation, and transfection, including endotoxin-sensitive cells.

Application

GenElute™ HP Endotoxin-Free Plasmid Megaprep Kit has been used to purify plasmid DNA.
High-quality, endotoxin-free DNA is ready for immediate use in downstream applications such as restriction digestion, ligation, sequencing, PCR, transformation, and transfection, including endotoxin-sensitive cells.

Features and Benefits

  • Up to 5 mg of high-copy plasmid DNA with endotoxin levels of ≤0.1 EU/μg
  • Only 90 minutes from pelleted cells to purified plasmid
  • Vacuum format with no ethanol precipitation required

Other Notes

For additional information, please see www.sigma-aldrich.com/genelutehp.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT RE 1 Inhalation - STOT SE 3

Target Organs

Central nervous system, Lungs

Storage Class Code

3 - Flammable liquids

Flash Point(F)

77.0 °F - closed cup

Flash Point(C)

25 °C - closed cup

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Immunogenic Salivary Proteins of Triatoma infestans: Development of a Recombinant Antigen for the Detection of Low-Level Infestation of Triatomines
Alexandra Schwarz
PLoS ONE, e532-e532 (2009)
Functionalization of poly (amidoamine) dendrimers with hydrophobic chains for improved gene delivery in mesenchymal stem cells
Jose Santos
Journal of Controlled Release : Official Journal of the Controlled Release Society (2010)

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