Anti-Nerve Growth Factor 2.5S, catalog number N6655, is produced in rabbits using immunogen 2.5S nerve growth factor purified from mouse submaxillary glands.
N6655
Anti-Nerve Growth Factor 2.5S antibody produced in rabbit
fractionated antiserum, buffered aqueous solution
Synonym(s):
Anti-NGF-2.5S
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0.1 ML
$264.00
About This Item
Recommended Products
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
fractionated antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
mouse, human
technique(s)
Ouchterlony double diffusion: suitable
western blot: 1:2,000
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... NGF(4803)
mouse ... Ngf(18049)
General description
Nerve growth factor (NGF) belongs to the family of neurotrophic factors. It is located at 1p13.2 on the human chromosome. There are two molecular forms of NGF, 7S NGF and 2.5S NGF, that have identical neurotrophic effect on neurons.
Specificity
May be used in monitoring for NGF (2.5S, β and 7S).
Immunogen
NGF-2.5S from male mouse submaxillary glands.
Application
Anti-Nerve Growth Factor 2.5S antibody may be used for immunoblotting and dot blot at a minimum antibody working dilution of 1:2000. Antibody dilution of 1:1000 was used for immunoblotting using extracts of nucleus raphe magus tissue of rats. The antibody was used for immunofluorescence of mouse astrocytes and for neutralization assays.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Western Blotting (1 paper)
Immunofluorescence (1 paper)
Western Blotting (1 paper)
Biochem/physiol Actions
Mutations in this gene leads to hereditary sensory and autonomic neuropathies class five (HSAN-V).
Physical form
Solution in phosphate buffered saline.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Is this antibody N6655 anti-NGF 2.5S against targeting NGFR?
1 answer-
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How does the storage temperature relate to shipping conditions?
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The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
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In western blot application, what size protein is recognized by Product N6655, Anti-Nerve Growth Factor 2.5S antibody produced in rabbit?
1 answer-
Product N6655, Anti-Nerve Growth Factor 2.5S antibody produced in rabbit, will recognize a 30 kD protein on western blot.
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