N6255
β-Nicotinamide adenine dinucleotide phosphate–Agarose
saline suspension
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About This Item
MDL number:
UNSPSC Code:
23151817
Recommended Products
form
saline suspension
extent of labeling
2-4 μmol per mL
matrix
Cross-linked 4% beaded agarose
matrix activation
cyanogen bromide
matrix attachment
ribose hydroxyls
matrix spacer
11 atoms (adipic acid dihydrazide)
storage temp.
2-8°C
Application
NADP-agarose is used for protein chromatography, affinity chromatography and nucleotide/coenzyme resins. NADP-agarose has been used to evaluate macroporous scaffolds for bone tissue engineering for biomedical applications.
Physical form
Suspension in 0.5 M NaCl containing 0.02% thimerosal
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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D Wyrambik et al.
European journal of biochemistry, 97(2), 503-509 (1979-07-01)
Isoenzyme 2 of cinnamyl-alcohol dehydrogenase from soybean suspension cultures was purified about 3800-fold to apparent homogeneity by an improved purification procedure involving biospecific elution of the enzyme from a NADP+-agarose column. On sodium dodecylsulfate gels the dehydrogenase showed only one
J A Puértolas et al.
Acta biomaterialia, 7(2), 841-847 (2010-08-17)
There is an acknowledged need for shaping 3-D scaffolds with adequate porosity and mechanical properties for biomedical applications. The mechanical properties under static and cyclic compressive testing of dense and designed porous architecture bioceramic scaffolds based on the biphasic calcium
Sreelatha T Reddy et al.
Protein expression and purification, 26(2), 290-300 (2002-10-31)
Mannose 6-phosphate receptors (MPRs) form essential components of the lysosomal enzyme targeting system by binding newly synthesized acid hydrolases with high (nM) affinity. We report the use of Pichia pastoris as a host to efficiently express the extracytoplasmic ligand-binding domain
M R Prasad et al.
Archives of biochemistry and biophysics, 237(2), 535-544 (1985-03-01)
The present study describes the solubilization and purification of a NADPH-specific trans-2-enoyl-CoA reductase from rat liver microsomes. The final preparation was purified to near homogeneity and had a minimal molecular weight of 51,000 +/- 2,000, as judged by sodium dodecylsulfate
C Andriamampandry et al.
The Biochemical journal, 334 ( Pt 1), 43-50 (1998-08-07)
The gamma-hydroxybutyrate biosynthetic enzyme succinic semialdehyde reductase (SSR) was purified to homogeneity from rat brain. Peptides were generated by tryptic cleavage and sequenced. PCR primers were designed from the amino acid sequences of two of the peptides showing a similarity
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