N214

Neutrophil Activating Peptide-2 human

>97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(s): NAP-2
• MDL number: MFCD00241917
• UNSPSC Code: 51111800

Properties

• biological source: human
• recombinant: expressed in E. coli
• Assay: >97% (SDS-PAGE)
• form: lyophilized powder
• potency: 0.1-0.3 μg/mL ED₅₀ (cytochalasin B treated human neutrophils); 0.1-0.5 ng/mL ED₅₀ (BaF/3 hCXCR-2 transfected cells)
• mol wt: ~7.6 kDa
• storage condition: avoid repeated freeze/thaw cycles (Storage in "frost-free" freezers is not recommended)
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• UniProt accession no.: P02775
• storage temp.: −20°C
• Gene Information: human ... PPBP(5473)

Description

Biochem/physiol Actions

Neutrophil Activating Peptide-2, a member of the CXC chemokine family, chemoattracts and activates neutrophils and also binds CXCR-2. NAP-2 is proteolytically processed C-terminal fragments of platelet basic protein (PBP), which is found in the α-granules of human platelets.

Physical form

Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% trifluoroacetic acid containing 0.5 mg bovine serum albumin.

Analysis Note

The biological activity is measured by the induction of myeloperoxidase release from cytochalasin B treated human neutrophils.