MBD0051

Yeast FISH probe-Cy3

Probe for fluorescence in situ hybridization (FISH), 20µM in water

• Synonym(s): PF2-Cy3 probe
• UNSPSC Code: 12352200
• NACRES: NA.54

Properties

• Quality Level: 200
• technique(s): FISH: suitable
• fluorescence: λ_ex 550 nm; λ_em 570 nm (Cy3)
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

FISH (Fluorescent in Situ Hybridization) is based on the hybridization of fluorescently labeled oligonucleotide probes to a specific complementary DNA or RNA sequence in whole and intact cells. Microbial FISH allows the visualization, identification, and isolation of bacteria due to recognition of ribosomal RNA also in unculturable samples.

FISH can serve as a powerful tool in the microbiome research field by allowing the observation of native microbial populations in diverse microbiome environments, such as samples from human origin (e.g., blood and other tissues), microbial ecology (biofilms, aquatic systems) and plants. It is strongly recommended to include positive and negative controls in FISH assays to ensure specific binding of the probe of interest and appropriate protocol conditions. We offer positive (MBD0032/33) and negative control probes (MBD0034/35) that accompany the specific probe of interest.

The yeast FISH (PF2-Cy3) probe specifically binds to a wide range of yeast species. Examples include Candida species that are common causes of bloodstream infections in humans, common environmental yeasts, and model organisms (Saccharomyces) in the phylum Ascomycota. The probe offered here was tested on 5 strains belonging to: Candida albicans, Candida boidinii, Candida glabrata, Saccharomyces cerevisiae, and Schizosaccharomyces pombe. As a negative control, each slide contained fixed Proteus mirabilis bacterial cells in separate wells, to which the yeast FISH probe did not bind, confirming its specificity. For each experiment, an additional slide was stained with Eubacteria FISH probe - Cy3 (MBD0033). The Eubacteria FISH probe only stained the bacterial Proteus cells.

The yeast FISH (PF2) probe sequence was shown to specifically identify the yeast microbiome from various samples: murine intestine, blood samples and in environmental samples.

Application

Yeast FISH probe-Cy3 is suitable to use as a probe for fluorescence in- situ hybridization (FISH) to specifically recognize yeast cells.

Features and Benefits

• Visualize, identify, and isolate yeast cells. Observe native yeast populations in a variety of samples.
• Specific, sensitive, and robust identification of yeasts in bacterial mixed population.
• FISH can complement PCR based detection methods by avoiding contaminant bacteria detection.
• Provides information on yeast morphology.
• Identifies yeasts in clinical samples.
• The ability to detect yeasts in their natural habitat is an essential tool for studying host-microbiome interaction.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable