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MAK006

Sigma-Aldrich

Urea Assay Kit

sufficient for 100 colorimetric tests

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

storage temp.

−20°C

General description

The new Urea Assay Kit, MAK471, is now available! Urea is the major end product of nitrogen metabolism in most animals and is produced in a series of reactions in the liver called the urea cycle. In the urea cycle, ammonia is converted to urea, which is carried by blood to the kidneys for elimination from the body. High levels of urea in the blood may indicate renal failure. Urea levels may also be elevated in response to treatment with certain drugs such as corticosteroids or in response to decreased kidney filtration due to dehydration or congestive heart failure. Decreased blood urea levels can occur in response to liver disease or malnutrition.

Application

Urea assay kit has been used to measure the concentration of urea.

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the measurement of urea concentration in a variety of samples such as serum, plasma, and urine, etc.

Principle

In this assay, Urea concentration is determined by a coupled enzyme reaction, which results in a colorimetric (570 nm) product, proportional to the Urea present.

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Pictograms

Health hazardExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

10 - Combustible liquids

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Inhibition of the RhoGTPase Cdc42 by ML141 enhances hepatocyte differentiation from human adipose-derived mesenchymal stem cells via the Wnt5a/PI3K/miR-122 pathway: impact of the age of the donor.
Chaker D, et al.
Stem Cell Research & Therapy, 9(1), 167-167 (2018)
Protein tyrosine phosphatase 1B deficiency in podocytes mitigates hyperglycemia-induced renal injury.
Ito Y, et al.
Metabolism, Clinical and Experimental, 76, 56-69 (2017)
Kate Smith-Jackson et al.
The Journal of clinical investigation, 129(3), 1061-1075 (2019-02-05)
Atypical hemolytic uremic syndrome (aHUS) is frequently associated in humans with loss-of-function mutations in complement-regulating proteins or gain-of-function mutations in complement-activating proteins. Thus, aHUS provides an archetypal complement-mediated disease with which to model new therapeutic strategies and treatments. Herein, we
Sriram Ravindran et al.
Cardiovascular drugs and therapy, 31(5-6), 511-524 (2017-10-02)
Sodium thiosulfate (STS) has of late been proven efficacious in models of urolithiasis and vascular calcification. However, its cardiovascular effects on ischemia reperfusion injury (IR) have not been revealed. Being an antioxidant and calcium chelator, it is assumed to play
Nitrogen excretion: three end products, many physiological roles.
Wright P A.
The Journal of Experimental Biology, 198(2), 273-281 (1995)

Protocols

Assay protocol for the colorimetric detection of Hydroxyproline in biological samples using the Hydroxypoline assay kit.

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