M9400
β-Mannosidase from Helix pomatia
5-30 units/mL, ammonium sulfate suspension, crude extract
Synonym(s):
β-D-Mannoside mannohydrolase
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.32
Recommended Products
form
ammonium sulfate suspension
quality
crude extract
mol wt
94 kDa
concentration
5-30 units/mL
foreign activity
β-N-acetylglucosaminidase and α-mannosidase <1.0%
storage temp.
2-8°C
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General description
β-Mannosidase is a lysosomal enzyme that belongs to the glycosyl hydrolase family 2.
Application
β-Mannosidase from Helix pomatia has been used:
- in the digestion of glycans for enzymatic analysis
- in the treatment of labeled monogalactosyl Manno-oligosaccharides
- in non-mammalian glycosidase inhibition assays
Biochem/physiol Actions
β-Mannosidase participates in cleaving the β(1–4)-linked mannose located at the nonreducing end of N-glycosylated proteins. It is involved in the polysaccharide degradation pathway. In humans, mutations in the gene are associated with β-mannosidosis, a lysosomal storage disease. Low levels of β-Mannosidase due to mutations also lead to nystagmus, an eye disorder associated with involuntary eye movements.
Unit Definition
One unit will hydrolyze 1 μmole of p-nitrophenyl-β-D-mannopyranoside to p-nitrophenol and D-mannopyranoside per min at pH 4.0 at 25°C.
Physical form
Suspension in 3.0 M (NH4)2SO4 containing 10 mM sodium acetate, pH approx. 4.0
inhibitor
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Barbora Fliedrová et al.
Protein expression and purification, 85(2), 159-164 (2012-08-14)
β-Mannosidase (EC 3.2.1.25) is an exoglycosidase specific for the hydrolysis of terminal β-linked mannoside in various sugar chains. cDNA corresponding to the β-mannosidase gene was cloned from Aspergillus niger, sequenced, and expressed in the yeast Pichia pastoris. The β-mannosidase gene
Sunghoon Park et al.
Protein and peptide letters, 20(1), 107-114 (2012-07-14)
Sequence alignments and homology modeling of Pyrococcus furiosus thermostable glycosidase (PFTG) showed that the residue 150 is conserved as tryptophan in β-glycosidase and in other related enzymes such as β- mannosidase and β-galactosidase. To elucidate the relationship between the substrate
Roneel Prakash et al.
Journal of plant physiology, 169(12), 1125-1133 (2012-06-05)
Cell walls of tomato fruit contain hemicellulosic mannans that may fulfill a structural role. Two populations were purified from cell walls of red ripe tomato tissue and named galactoglucomannan-glucuronoxylan I and II (GGM-GX I and II), respectively. Both polysaccharides not
P Wongputtisin et al.
Journal of applied microbiology, 113(4), 798-806 (2012-07-14)
To screen and select the Bacillus spp. from Tua-nao of northern Thailand for fermented corticate soybean meal (FCSBM) production. After isolation of Bacillus spp. from Tua-nao was carried out, cellulase, hemicellulases (i.e., β-mannanase and xylanase) and phytase production by isolated
A A Klyosov et al.
Carbohydrate research, 352, 65-69 (2012-03-23)
Statistical modeling was applied for describing structural features of β-(1→4)-D-galactomannans. According to the model suggested theoretical ratios of limiting degrees of locust bean, tara gum and guar gum galactomannan conversions by two β-(1→4)-mannanases of different origin (Myceliophthora thermophila and Trichoderma
Articles
Instructions for working with enzymes supplied as ammonium sulfate suspensions
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