L3759
Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia)
biotin conjugate, lyophilized powder
Synonym(s):
Bandeirea simplicifolia agglutinin, BS-I
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
Recommended Products
conjugate
biotin conjugate
form
lyophilized powder
potency
<250 μg per mL agglutination activity (using human blood group A erythrocytes.)
composition
Protein, ~85% E1%/280
extent of labeling
~5 mol biotin per mol protein
storage temp.
2-8°C
Looking for similar products? Visit Product Comparison Guide
Application
Lectin from Bandeiraea simplicifolia (Griffonia simplicifolia) has been used:
- for highlighting murine endothelial cells
- to identify fetal capillaries in endothelial immunohistochemical staining, conjugated with biotin
- to detect tumor total vessels
Biochem/physiol Actions
BS-I has a major affinity for terminal α-D-galactosyl residues with a secondary affinity for terminal N-acetyl-α-D-galactosaminyl residues.
Other Notes
BS-I is a tetrameric lectin consisting of two types of subunits designated A and B. There are five BS-I isolectins with different subunit composition: BSI-B4, BSI-AB3, BSI-A2B2, BSI-A3B and BSI-A4. BSI-B4 is blood group B specific and has an exclusive affinity for terminal α-D-galactosyl residues, whereas BSI-A4 has blood group A specificity and has a major affinity for terminal N-acetyl-α-D-galactosaminyl residues.
Physical form
Contains sodium citrate
Analysis Note
Agglutination activity is expressed in μg/mL and is determined from serial dilutions of a 1 mg/mL solution using phosphate buffered saline, pH 6.8, containing, for each lectin, calcium, magnesium, and manganese at different concentrations. This activity is the lowest concentration to agglutinate a 2% suspension of appropriate erythrocytes after 1 hr incubation at 25 °C.
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Laura Schaad et al.
Scientific reports, 7, 41842-41842 (2017-02-09)
A detailed vascular visualization and adequate quantification is essential for the proper assessment of novel angiomodulating strategies. Here, we introduce an ex vivo micro-computed tomography (microCT)-based imaging approach for the 3D visualization of the entire vasculature down to the capillary
Yun Sook Kim et al.
The Journal of comparative neurology, 518(5), 687-698 (2009-12-25)
Transient receptor potential ankyrin 1 (TRPA1), responding to noxious cold and pungent compounds, is implicated in the mediation of nociception, but little is known about the processing of the TRPA1-mediated nociceptive information within the trigeminal sensory nuclei (TSN) and the
Intravitreal and subconjunctival melphalan for retinoblastoma in transgenic mice
Shah NV, et al.
Journal of Ophthalmology, 2014 (2014)
Suppressive effect of 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin on vascular remodeling that takes place in the normal labyrinth zone of rat placenta during late gestation
Ishimura R, et al.
Toxicological Sciences, 91(1), 265-274 (2006)
Merle M Krebber et al.
Journal of hypertension, 38(3), 489-503 (2019-10-28)
Left ventricular (LV) hypertrophy is the most common cardiac alteration in patients with chronic kidney disease (CKD). Normalization of hypertension in CKD patients receiving a healthy kidney allograft often reverses LV hypertrophy, but effects on LV fibrosis remain unclear. To
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service