Skip to Content
Merck
All Photos(1)

Documents

L020000

Sigma-Aldrich

TCA Deblock

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

UNSPSC Code:
41116105
NACRES:
NA.21

type

for DNA synthesis, for DNA synthesis
for DNA synthesis

storage condition

dry at room temperature

concentration

28-32 g/L (TCA content, titration)

impurities

≤200 ppm water content (Karl Fischer)

color

clear
clear

Looking for similar products? Visit Product Comparison Guide

Preparation Note

Trichloroacetic acid / Dichloromethane 3/100 (v/v)

Other Notes

Deblock solution containing trichloroacetic acid (TCA) in dichloromethane removes the dimethoxytrityl (DMT) protecting group from the 5′hydroxyl moiety of nucleotides already incorporated into the growing nucleic acid, prior to the addition of the next phosphoramidite. Removal of the DMT allows the unprotected 5′hydroxyl moiety to react with a new phosphoramidite in a subsequent extension reaction.

Signal Word

Danger

Hazard Classifications

Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Central nervous system, Respiratory system

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 2

Flash Point(F)

212.0 °F - closed cup

Flash Point(C)

100 °C - closed cup

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service