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GE17-0690-01

Lysine-Sepharose™ 4B

Cytiva 17-0690-01, pack of 15 g

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About This Item

UNSPSC Code:
13111000

ligand

L-lysine

packaging

pack of 15 g

manufacturer/tradename

Cytiva 17-0690-01

matrix

4% agarose

particle size

45-165 μm

average diameter

90 μm

cleaning in place

2-11

working range

2-11

storage temp.

2-8°C

General description

Lysine Sepharose™ 4B media with immobilized amino acid lysine is mainly used for isolation of plasminogen and plasminogen activator. The ε-amino and α-carboxyl groups on lysine is free after immobilization and interact with a range of biomolecules.

Application

Lysine Sepharose™ 4B is L-lysine immobilized to Sepharose™ 4B by the cyanogen bromide method. L-lysine is coupled via its α-amino group, leaving both the ε-amino and α-carboxyl groups free to interact with sample substances during chromatography. It is designed for purification of molecules with biospecific or charge dependent affinity for L-lysine. Lysine Sepharose™ 4B is a group specific adsorbent and has been used for isolation of plasminogen and plasminogen activator, separation of ribosomal RNA (rRNA) and purification of doublestranded DNA. The precise mechanism by which separation occurs is not fully understood but both electrostatic and stereospecific effects may contribute to the separation, depending upon the application.

Features and Benefits

  • Mainly used for isolation of plasminogen and plasminogen activator, separation of ribosomal RNA (rRNA) and purification of doublestranded DNA.
  • It is designed for purification of molecules with biospecific or charge dependent affinity for L-lysine.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

Storage Class Code

13 - Non Combustible Solids

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