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G2150

Sigma-Aldrich

L-Glutamine solution

200 mM, Hybri-Max™, sterile-filtered, suitable for hybridoma

Synonym(s):

L-Glutamine for cell culture

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About This Item

CAS Number:
56-85-9
MDL number:
MFCD00008044
UNSPSC Code:
12352209
eCl@ss:
32160406
PubChem Substance ID:
24895088
NACRES:
NA.75

grade

Hybri-Max™

sterility

sterile-filtered

Assay

≥99% (TLC)

form

solution

technique(s)

cell culture | hybridoma: suitable

impurities

endotoxin, tested

color

clear colorless

shipped in

dry ice

storage temp.

−20°C

SMILES string

N[C@@H](CCC(N)=O)C(O)=O

InChI

1S/C5H10N2O3/c6-3(5(9)10)1-2-4(7)8/h3H,1-2,6H2,(H2,7,8)(H,9,10)/t3-/m0/s1

InChI key

ZDXPYRJPNDTMRX-VKHMYHEASA-N

Gene Information

rat ... Ggt1(116568)

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General description

L-glutamine is an essential amino acid that is a crucial component of culture media that serves as a major energy source for cells in culture. L-glutamine is very stable as a dry powder and as a frozen solution. In liquid media or stock solutions, however, L-glutamine degrades relatively rapidly. Optimal cell performance usually requires supplementation of the media with L-glutamine prior to use.

Application

The L-Glutamine solution has been used as a supplement in Roswell Park Memorial Institute (RPMI)-1640 medium to culture human leukemia monocytic cell line for intracellular assay and splenocytes for in vitro activation of CD4+ T cells. It has also been used as a supplement to the hybridoma medium used for primary screening for antigen binding and hybridoma cloning.

Preparation Note

Prepared in cell culture grade water.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Production of human antibodies from transgenic mice.
C Geoffrey Davis et al.
Methods in molecular biology (Clifton, N.J.), 248, 191-200 (2004-02-19)
Marina K Balabekova et al.
Drug and chemical toxicology, 44(3), 277-285 (2019-03-09)
Heavy metal pollution is rapidly increasing in the environment. It has been shown that exposure to vanadium and chromium is able to alter the immune response. Nevertheless, the mechanisms by which these metal pollutants mediate their immunomodulatory effects are not
Abraham L Moure et al.
Journal of medicinal chemistry, 63(9), 4732-4748 (2020-04-11)
Screening of a GSK-proprietary library against intracellular Mycobacterium tuberculosis identified 1, a thioalkylbenzoxazole hit. Biological profiling and mutant analysis revealed that this compound is a prodrug that is bioactivated by the mycobacterial enzyme MymA. A hit-expansion program including design, synthesis
Goonho Park et al.
Cell reports, 31(13), 107839-107839 (2020-07-02)
Amyloid β-protein (Aβ) toxicity is hypothesized to play a seminal role in Alzheimer's disease (AD) pathogenesis. However, it remains unclear how Aβ causes synaptic dysfunction and synapse loss. We hypothesize that one mechanism of Aβ-induced synaptic injury is related to
Glutamine and antioxidants in critically ill patients.
Daren Heyland et al.
The New England journal of medicine, 369(5), 484-485 (2013-08-02)
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Articles

L-Glutamine in Cell Culture

Importance and uses of glutamine in hybridoma and mammalian cell culture

Chromatograms

application for HPLC

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