CELLMM2
FLAG® M Purification Kit
For Mammalian expression systems.
Synonym(s):
Anti-ddddk, Anti-dykddddk
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
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Application
The FLAG Purification Kit utilizes CelLytic™ Reagents for rapid and efficient cell lysis and protein extraction and ANTI-FLAG® M2 affinity gel for affinity purification of active FLAG fusion proteins.
Learn more product details in our FLAG® application portal.
Learn more product details in our FLAG® application portal.
Features and Benefits
- Includes ready to use reagents, columns, and a detailed protocol for affinity purification of FLAG fusion proteins.
- ANTI-FLAG M2 Affinity Gel allows efficient binding of FLAG fusion proteins without the need for preliminary steps and calibrations.
- Two alternatives for efficient elution conditions (by acidic conditions or by competition with FLAG peptide).
Packaging
Sufficient for 3-5 uses of 1-ml affinity purification column.
Other Notes
Kit contains CelLytic™ M for cell lysis and protein extraction from Mammalian cells.
Legal Information
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
CelLytic is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Kit Components Also Available Separately
Product No.
Description
SDS
- C2978CelLytic™ M, Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization.SDS
- SAE0194Purified 3xFLAG® peptide, ≥95% (HPLC), lyophilized powderSDS
- A2220ANTI-FLAG® M2 Affinity Gel, purified immunoglobulin, buffered aqueous glycerol solutionSDS
- C2103Chromatography columns, general-purpose, volume 10 mL, Overall H 5 in.SDS
Storage Class Code
10 - Combustible liquids
Certificates of Analysis (COA)
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