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biological source
Rat x Rat Hybridoma
ImmunoDon
DA rat spleen
Myeloma
Y3.AG.1.2.3
Reactivity
Human CD7
technique(s)
cell culture | mammalian: suitable
isotype
IgG2b
shipped in
dry ice
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Cell Line Origin
Monoclonal antibody human CD28
Cell Line Description
Rat hybridoma producing antibody against human CD7. Cambridge Enterprise Reference number AB69:www.enterprise.cam.ac.uk.
Immunogen
Human peripheral blood mononuclear cells
Culture Medium
IDMEM (I3390) + 2 mM L-Glutamine (G7513) + 5% FBS / FCS (F2442). Where the cells are grown under static conditions, 0.02% EDTA, Trypsin (T4424) or mechanical agitation maybe required to dislodge them from the plastic.
Subculture Routine
Maintain cultures between 3-9 x 105 cells/ml. 5% CO2; 37 °C. Saturation density 9 x 105/ml.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
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