C8239

Anti-α-N-Catenin antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• MDL number: MFCD05665181
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 102 kDa
• species reactivity: mouse
• technique(s): microarray: suitable; western blot: 1:1,000 using cytosolic fraction of mouse brain
• UniProt accession no.: Q61301
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: mouse ... Ctnna2(12386)

Description

General description

The catenins (α, β, γ) are cytoplasmic proteins found in varying developing and adult tissues. α- N-catenin is a cadherin-binding protein, that plays a crucial role in cadherin-mediated cell-cell adhesion. It is widely expressed in the nervous system. α-Catenin (CAP102, 102 kDa), originally described as an E-cadherin associated protein, has been shown to associate with N-cadherin and P-cadherin. Within its conserved region α-catenin shows 30% identity to vinculin. There are at least two subtypes of α-catenin: α-E-catenin and α-N-catenin (102 kDa). The predominant form is α-E-catenin. Alternative spliced forms of α-N-catenin include α-N-catenin I and α-N-catenin II. The ratio of the two isoforms changes during development: isoforms II is more abundant than isoform I in early embryonic development, whereas isoform I is predominant in the adult stage.

Immunogen

synthetic peptide corresponding to a region located near the N-terminus of human α-N-catenin (amino acids 171-186) conjugated to KLH. This sequence is identical in mouse and chicken α-N-catenin. It is not found in α-E-catenin, β-catenin, and γ-catenin.

Application

α-N-Catenin antibody is suitable for microarray and western blot at a dilution of 1:1,000 by using cytosolic fraction of mouse brain.

Biochem/physiol Actions

Catenins bind directly or indirectly to the conserved cytoplasmic tail domain of the cell adhesion cadherins. The association of catenins to cadherins produces a complex, which is linked to the actin filament network. Catenins/cadherin complexes play an important role in mediating cell adhesion, transduction of cell-cell contact positional signals to the cell interior, and may play a crucial role in cell differentiation. The expression of α-N-catenin is more restricted and this form predominates in the brain and is localized at synaptic junctions.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves