C3660

Complement C9 from human serum

≥150,000 C9H50 units/mg (using C9 deficient serum)

• CAS Number: 80295-59-6
• MDL number: MFCD00130849
• UNSPSC Code: 12352202
• NACRES: NA.61

Properties

• biological source: human
• Quality Level: 200
• Assay: ≥85% (SDS-GE)
• form: solution
• specific activity: ≥150,000 C9H50 units/mg (using C9 deficient serum)
• UniProt accession no.: P02748
• application(s): cell analysis
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... C9(735)

Description

Application

Complement C9 from human serum is an essential part of the terminal complement system. It has been a topic in cancer research, where extracellular phosphorylation by ecto-PK of K562 cells on serine residues may serve as a protective mechanism against complement in tumor cells. Additionally, it can be used as a biomarker (in fucosylated form) for squamous cell lung cancer, as patients tend to show elevated levels of C9 protein.

Biochem/physiol Actions

Complement component C9 is the final component of the membrane attack complex (MAC) responsible for cell lysis by complement. CD59 binds to both C8 and C9 and prevents assembly of an active MAC, protecting cells against lytic activity.[1]

Quality

Functionally pure by a sensitive hemolytic assay using deficient sera.

Physical form

Supplied as a solution in phosphate buffered saline, pH 7.2.

Other Notes

View more information on the complement pathway at www.sigma-aldrich.com/enzymeexplorer

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable