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A8792

Sigma-Aldrich

Anti-Human IgG (whole molecule)−Peroxidase antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

HRP Rabbit Anti-Human IgG (whole molecule)

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2 ML
$460.00

$460.00

Estimated to ship on11 April 2025

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2 ML
$460.00

About This Item

MDL number:
MFCD00162456
UNSPSC Code:
12352203
NACRES:
NA.46

$460.00

Estimated to ship on11 April 2025

Request a Bulk Order

biological source

rabbit

Quality Level

200

conjugate

peroxidase conjugate

antibody form

IgG fraction of antiserum

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

human

technique(s)

direct ELISA: 1:40,000
dot blot: 1:80,000 (chemiluminescent)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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General description

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders.
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. IgG is usually found as a monomer. IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. About 70 percent of the total immunoglobulin consists of IgG.

Specificity

Rabbit Anti-Human IgG (whole molecule)-Peroxidase antibody binds to human IgG.

Immunogen

purified human IgG

Application

Anti-Human IgG (whole molecule)-Peroxidase antibody produced in rabbit has been used in:
enzyme-linked immunosorbent assay (ELISA)[1]
western blotting
microarray analysis
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (1 paper)
Western Blotting (1 paper)

Biochem/physiol Actions

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000416014
0000416014
0000389403
0000389403
0000388872

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Rajika Lasanthi Dewasurendra et al.
BMC infectious diseases, 17(1), 49-49 (2017-01-11)
Sri Lanka achieved the WHO certificate as a malaria free country in September 2016, thus monitoring of malaria transmission using sensitive and effective tools is an important need. Use of age-specific antibody prevalence as a serological tool to predict transmission
Jenny Calow et al.
mAbs, 8(8), 1498-1511 (2016-10-30)
Antibody glycosylation is a key parameter in the optimization of antibody therapeutics. Here, we describe the production of the anti-cancer monoclonal antibody rituximab in the unicellular ciliate, Tetrahymena thermophila. The resulting antibody demonstrated enhanced antibody-dependent cell-mediated cytotoxicity, which we attribute
Mônica Franca et al.
Journal of applied oral science : revista FOB, 15(3), 213-219 (2007-06-01)
Periodontitis is a chronic disease that results from an interaction of a mixed bacterial challenge and the host response. The purposes of this study were to evaluate the IgG serum levels to Porphyromonas gingivalis antigens by ELISA in individuals with
Cross-reactions between Toxocara canis and Ascaris suum in the diagnosis of visceral larva migrans by western blotting technique
Nunes CM, et al.
Revista Do Instituto De Medicina Tropical De Sao Paulo, 39(5), 253-256 (1997)
Carlos Sánchez-Ovejero et al.
PLoS neglected tropical diseases, 14(11), e0008892-e0008892 (2020-12-01)
Cystic echinococcosis (CE) is a neglected zoonotic disease caused by Echinococcus granulosus sensu lato. Diagnosis and monitoring of CE rely primarily on imaging while serology is used as a confirmatory test. However, imaging is not always conclusive and currently available
View All Related Papers

Questions

  1. what is the recommended dilution for western blotting

    1 answer

    1. The Western Blot picture is from the article in link below. However, It does not mention how the product is diluted:
      https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0026032&type=printable

      Below are links to articles that have cited this antibody in Western Blot. Various dilutions are used. The end-user may titrate the product to find the best dilution for their application. The end-user may try something like 1:1000, 1:5000, 1:10,000, 1:20,000, etc.:

      https://www.researchgate.net/profile/Yuriy-Kit/publication/303736655_Identification_of_the_Unique_Properties_of_IgGs_and_their_Heavy_chains_in_Blood_serum_of_Multiple_Sclerosis_Patients/links/574ff60608aef199238f01e4/Identification-of-the-Unique-Properties-of-IgGs-and-their-Heavy-chains-in-Blood-Serum-of-Multiple-Sclerosis-Patients.pdf

      https://www.scielo.br/j/jaos/a/pBt9nVwJydK6Qz3qwMrNLrc/?lang=en

      https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2020.612781/full

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