BAPTA-AM is soluble in dimethyl sulfoxide (DMSO) at 25 mg/mL. It is also soluble in acetonitrile at 0.5 mg/mL. It is best to use anhydrous DMSO, since it is known that moisture uptake by the DMSO can adversely affect the quality of the BAPTA-AM.
A1076
BAPTA-AM
≥95% (HPLC), powder, intracellular Ca²⁺ store chelator
Synonym(s):
1,2-Bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid tetrakis(acetoxymethyl ester)
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25 MG
$476.00
About This Item
Empirical Formula (Hill Notation):
C34H40N2O18
CAS Number:
Molecular Weight:
764.68
Beilstein:
7458534
MDL number:
UNSPSC Code:
12352200
PubChem Substance ID:
NACRES:
NA.77
Recommended Products
Product Name
BAPTA-AM, ≥95% (HPLC)
Quality Level
Assay
≥95% (HPLC)
form
powder
storage temp.
−20°C
SMILES string
CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)c1ccccc1OCCOc2ccccc2N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O
InChI
1S/C34H40N2O18/c1-23(37)47-19-51-31(41)15-35(16-32(42)52-20-48-24(2)38)27-9-5-7-11-29(27)45-13-14-46-30-12-8-6-10-28(30)36(17-33(43)53-21-49-25(3)39)18-34(44)54-22-50-26(4)40/h5-12H,13-22H2,1-4H3
InChI key
YJIYWYAMZFVECX-UHFFFAOYSA-N
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General description
Application
BAPTA-AM has been used :
- to reduce the elevation of cleaved caspase-3[3]
- to block cell death, inhibit ROS (reactive oxygen species) production and inhibit caspase-8 activation[4]
- to exclude the effect of changes in intracellular Ca2+ ([Ca2+]i) concentration during seizure induction[5]
- to eliminate the increase of [Ca2+]i induced by 6-hydroxydopamine (6-OHDA)[6]
Biochem/physiol Actions
Selective chelator of intracellular Ca2+ stores.
Linkage
Membrane-permeable form of BAPTA.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Hyperactivation of the mammalian degenerin MDEG promotes caspase-8 activation and apoptosis
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The Journal of Biological Chemistry, 288(5), 2952-2963 (2013)
The membrane permeable calcium chelator BAPTA-AM directly blocks human ether a-go-go-related gene potassium channels stably expressed in HEK 293 cells
Tang Q, et al.
Biochemical Pharmacology, 74(11), 1596-1607 (2007)
Blockade of RyRs in the ER Attenuates 6-OHDA-induced calcium overload, cellular hypo-excitability and apoptosis in dopaminergic neurons
Huang L, et al.
Frontiers in Cellular Neuroscience, 11, 52-52 (2017)
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M-calpain activation facilitates seizure induced KCC2 down regulation
Wan L, et al.
Frontiers in Molecular Neuroscience, 11, 287-287 (2018)
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In what solvents is BAPTA-AM, Product A1076, soluble?
1 answer-
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What is BAPTA-AM, Product A1076, and how is it used?
1 answer-
BAPTA-AM is a cell-permeant acetoxymethyl ester derivative of BAPTA (Product No. A4926), which is used as a chelator of intracellular Ca2+ stores. The acetoxymethyl ester derivative, which is an ester of the carboxylic acid groups in BAPTA, results in an uncharged molecule which allows it to penetrate cell membranes. Inside the cell, these groups are cleaved by nonspecific esterase activity resulting in the free acid form.
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How would you recommend that I store my BAPTA-AM, Product A1076, solution?
1 answer-
Stock solutions of 1-10 mM can be prepared in anhydrous DMSO, divided into single-use aliquots, and stored desiccated and frozen at -20°C.
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What is the typical working concentration for BAPTA-AM, Product A1076?
1 answer-
The typical final working concentrations for cell loading is in the range of 1 to 10 micromolar. It is best to find literature references dealing with your application for more information on the amounts to use.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What are the shipping and storage temperatures for BAPTA-AM, Product A1076?
1 answer-
BAPTA-AM is shipped at ambient temperature. The recommended storage temperature, once received, is -20 °C.
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