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biological source
human
reprogramming method
episomal
description
age (N/A)
manufacturer/tradename
EBiSC™
gender
female
growth mode
adherent (pluripotent)
technique(s)
cell culture | stem cell: suitable
relevant disease(s)
Alzheimer′s disease
shipped in
dry ice
storage temp.
−196°C
General description
Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.
The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.
Cell Line Origin
Depositor
Bioneer
Bioneer
Cell Line Description
Derivation
Primary cell type: -
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
Lin28
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Notes on reprogramming vector detection: refer to BIONi037-A
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Morphology:
02-06-2017 bioni037-a-1 apoe ko 16423.tif
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Cell line karyotype: 23
Karyotyping method: G-Banding
Image / Result
Genetic Modification
Disease/phenotype related modifications
Disease: Alzheimer′s disease
Type of modification: Gene knock-out
Gene: Apolipoprotein E
Chromosome location: 19q13.32
Delivery method: CRISPR-associated (CRISPR/Cas) System
Description: WB analyses reveals a knockout of the APOE gene
Primary cell type: -
Reprogramming method
Vector type: Non-integrating
Vector: Episomal
Gene list:
KLF4
Lin28
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Notes on reprogramming vector detection: refer to BIONi037-A
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Morphology:
02-06-2017 bioni037-a-1 apoe ko 16423.tif
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Cell line karyotype: 23
Karyotyping method: G-Banding
Image / Result
Genetic Modification
Disease/phenotype related modifications
Disease: Alzheimer′s disease
Type of modification: Gene knock-out
Gene: Apolipoprotein E
Chromosome location: 19q13.32
Delivery method: CRISPR-associated (CRISPR/Cas) System
Description: WB analyses reveals a knockout of the APOE gene
Linkage
Note: EAUA and CLIP must be completed before order fulfillment
Subculture Routine
Surface coating: Matrigel®/Geltrex®
Passage method: Enzyme-free cell dissociation / EDTA
Culture medium: Essential E8®
No. Vials banked (pre-EBiSC): 30
Passage method: Enzyme-free cell dissociation / EDTA
Culture medium: Essential E8®
No. Vials banked (pre-EBiSC): 30
Legal Information
E8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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