66540472
EDi019-A
human dermis (fibroblast)
Synonym(s):
EBiSC iPSC Line, Human iPSC, Induced Pluripotent Stem Cell Line, iPS Cell, iPSC
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About This Item
UNSPSC Code:
12352207
product name
EDi019-A, Human iPS Cell Line
biological source
human dermis (fibroblast)
Quality Level
reprogramming method
Sendai virus
description
age (N/A)
manufacturer/tradename
EBiSC™
gender
female
growth mode
adherent (pluripotent)
relevant disease(s)
none
shipped in
dry ice
storage temp.
−196°C
General description
Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.
The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.
Cell Line Origin
Depositor
University of Edinburgh
University of Edinburgh
Cell Line Description
Derivation
Primary cell type: Fibroblast of dermis
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Other:From the same donor: EDi019-B, EDi019-C
Characterization
Analysis of Undifferentiated Cells
Marker expression: SSEA-4(+)POU5F1 (OCT-4)(+)SSEA-1 (-)TRA 1-60 (+)
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Genotyping
Karyotyping
Passage number: 23
Cell line karyotype: NO autosomal or sex chromosome abnormalities were detected
Karyotyping method: Karyolite BoBs
Primary cell type: Fibroblast of dermis
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: Unknown
Other:From the same donor: EDi019-B, EDi019-C
Characterization
Analysis of Undifferentiated Cells
Marker expression: SSEA-4(+)POU5F1 (OCT-4)(+)SSEA-1 (-)TRA 1-60 (+)
Microbiology / Virus Screening
HIV 1: -
HIV 2: -
Hepatitis B: -
Hepatitis C: -
Mycoplasma: -
Genotyping
Karyotyping
Passage number: 23
Cell line karyotype: NO autosomal or sex chromosome abnormalities were detected
Karyotyping method: Karyolite BoBs
Linkage
Note: EAUA and CLIP must be completed before order fulfillment
Subculture Routine
Surface coating: Matrigel®/Geltrex®
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 21%
CO2 concentration: 5%
Culture medium: Essential E8®
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 21%
CO2 concentration: 5%
Culture medium: Essential E8®
Legal Information
E8 is a registered trademark of WIsconsin Alumni Research Foundation non-stock Corporation
EBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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