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F5415

Sigma-Aldrich

Ficoll® solution

Type 400, 20% in H2O

Synonym(s):

Ready-to-use density gradient media

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About This Item

CAS Number:
26873-85-8
MDL number:
MFCD00081599
UNSPSC Code:
12352207
NACRES:
NA.75

sterility

0.2 μm filtered

Quality Level

200

type

Type 400

form

liquid

concentration

20% in H2O

color

clear colorless

solubility

water: soluble

density

1.055-1.075 g/mL

application(s)

hematology
histology

storage temp.

2-8°C

Related Categories

General description

Ficoll® Solution is a highly branched polymer of sucrose, also known as polydisperse mixtures of dextran. Ficoll® molecules are inert and not re-absorbed by proximal tubules. The structure is open and deformable, and not a rigid sphere thereby becoming a preferred choice in “crowding” experiments.
Used in electrophoresis and as a hapten carrier. Most commonly used to prepare density gradients.

Application

Ficoll® has been used in


  • Isolation of cord blood mononuclear cells
  • Isolation of CD133 negative cells
  • Isolation and expansion of circulating endothelial progenitor cells.

Other Notes

A nonionic synthetic polymer of sucrose.

Legal Information

Ficoll is a registered trademark of Cytiva

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000347993
0000347993
0000347992
SLCQ8241
SLCM9158

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Circulation enrichment of functional endothelial progenitor cells by infantile phototherapy.
Siavashi V, et al.
Journal of Cellular Biochemistry, 118(2), 330-340 (2017)
Xiaoliang Wu et al.
Shock (Augusta, Ga.), 51(6), 718-724 (2018-08-07)
The aim of this study was to investigate the correlation between plasma sphingosine-1-phosphate (S1P) and ceramide concentrations in sepsis, and the possible mechanisms for altered expression. Plasma S1P and ceramide concentrations were measured by HPLC-ESI-MS/MS. HLA-DR (human leukocyte antigen-DR) expression
Vahid Siavashi et al.
Inhalation toxicology, 32(3), 131-140 (2020-04-22)
Background: Sulfur mustard (SM), also known as mustard gas, was first widely used in the Iraq-Iran. After SM exposure, the most prominent clinical signs are the development of extensive non-healing skin wounds and pulmonary signs, persisting over long time. Since
Juliane Schmidt et al.
PloS one, 6(3), e18184-e18184 (2011-03-30)
The characterization of factors contributing to the formation and development of surface-associated bacterial communities known as biofilms has become an area of intense interest since biofilms have a major impact on human health, the environment and industry. Various studies have
Analysis of Structural and Functional Glomerular Changes in Hyperglycemic Rats that Treated with Human Umbilical Cord Blood Derived CD133 Negative Ccells.
Feizi F, et al.
Physical Chemistry, 1(1) (2014)
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