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46640

Supelco

7-Fluorobenzofurazan-4-sulfonic acid ammonium salt

for HPLC derivatization, LiChropur™, ≥98.5% (HPLC)

Synonym(s):

4-Fluoro-7-sulfobenzofurazan ammonium salt, 7-Fluorobenz-2,1,3-oxadiazole-4-sulfonic acid ammonium salt, Ammonium 7-fluorobenzofurazan-4-sulfonate, SBD-F

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About This Item

Linear Formula:
C6H3FN2O4S · NH3
CAS Number:
84806-27-9
Molecular Weight:
235.19
Beilstein:
5199564
MDL number:
MFCD02682109
UNSPSC Code:
12000000
PubChem Substance ID:
329757223
NACRES:
NA.22
Pricing and availability is not currently available.

grade

derivatization grade ((HPLC))
for HPLC derivatization

Quality Level

100

Assay

≥98.5% (HPLC)

quality

LiChropur™

technique(s)

HPLC: suitable

fluorescence

λex 385 nm; λem 524 nm in 0.1 M borate pH 9.5 (after derivatization with glutathione)

storage temp.

2-8°C

SMILES string

N.OS(=O)(=O)c1ccc(F)c2nonc12

InChI

1S/C6H3FN2O4S.H3N/c7-3-1-2-4(14(10,11)12)6-5(3)8-13-9-6;/h1-2H,(H,10,11,12);1H3

InChI key

JXLHNMVSKXFWAO-UHFFFAOYSA-N

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Application

Fluorescent probe for thiols and pre-column labeling of biological thiols for HPLC

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

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Legal Information

LiChropur is a trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCH2941

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Fluorometric assay of thiols with fluorobenzoxadiazoles.
K Imai et al.
Methods in enzymology, 143, 67-75 (1987-01-01)
I Daskalakis et al.
Biomedical chromatography : BMC, 10(5), 205-212 (1996-09-01)
A sensitive HPLC-fluorescence method for determining total endogenous plasma homocysteine (Hcy), cysteine (Cys) and cysteinylglycine (Cys-Gly) following derivatization with ammonium 7-fluoro 2,1,3-benzoxadiazole-4-sulphonate (SBD-F) is described. Quantitation utilizes an internal standard, 2-mercaptoethylamine. The derivatization procedure has been optimized for concentration of
J B Ubbink
Seminars in thrombosis and hemostasis, 26(3), 233-241 (2000-09-30)
Analytical methods to measure plasma total homocyst(e)ine (tHcy) concentrations are reviewed. High-pressure liquid chromatography (HPLC) with fluorometric detection is the most widely used method to determine plasma tHcy concentrations. Both monobromobimane and ammonium 7-fluorobenzo-2-oxa-1,3-diazole-4-sulphonate (SBD-F) are popular thiol-specific fluorogenic agents
J Tanaka et al.
Journal of biochemistry, 127(4), 597-601 (2000-03-31)
Pseudomonas sp. 109 produces a unique lipase (LipL) which efficiently catalyzes intramolecular transesterification of omega-hydroxyesters to form macrocyclic lactones. The production of the enzymatically active LipL requires a specific molecular chaperon (LimL protein) together with a low-M(r) lipase-activation-factor (LAF) of
Total plasma homocysteine analysis by HPLC with SBD-F precolumn derivatization.
I Fermo et al.
Methods in molecular biology (Clifton, N.J.), 159, 237-244 (2000-10-29)
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