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ROAMYGLL

Roche

Amyloglucosidase

from Aspergillus niger

Synonym(s):

Aspergillus niger, disaccharidase-type a-glucosidase

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About This Item

Enzyme Commission number:
3.2.1.3 (BRENDA, IUBMB)
UNSPSC Code:
12352204

biological source

Aspergillus niger

Quality Level

100

form

lyophilized

specific activity

6 U/mg (approximately 6 U/mg lyophilizate at +25°C with glycogen as the substrate)
~6 units/mg protein (At 25 °C with glycogen as the substrate.)

mol wt

97 kDa

packaging

pkg of 3,500 U (11202367001)
pkg of 500 U (11202332001)

manufacturer/tradename

Roche

optimum pH

4.6-4.8

storage temp.

2-8°C

General description

Amyloglucosidase, a 1,4-α-D-glucan glucohydrolase, is a disaccharidase–type α-glucosidase. It is synthesized by several Aspergillus genus species. This exo-enzyme is one of the major industrial enzymes. The stability of amyloglucosidase can be increased by immobilization.

Specificity

Cleaves terminal glucoses that are α1,4- or α1,6-linked to an oligo- or polysaccharide of multiple glucose units. The product is D-glucose.

Application

Amyloglucosidase from Aspergillus niger is used for the determination of starch.

Biochem/physiol Actions

Amyloglucosidase from Aspergillus niger is capable of hydrolyzing the α-D-(1-4) and α-(1,6) glucosidic bonds of starch and malto-oligosaccharides. Amyloglucosidase is an extracellular enzyme that converts starch to dextrins and glucose. The enzyme is used in the starch-processing industry in the commercial production of D-glucose from corn syrup.

Preparation Note

Working concentration: Working concentration of conjugate depends on application and substrate.

The following concentrations should be taken as a guideline:
  • Western blot: 1 to 10 μg/ml


Working solution: Tris-buffered saline containing 0.1% Tween 20.

Storage conditions (working solution): 2 to 8 °C

Other Notes

For life science research only. Not for use in diagnostic procedures.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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High fructose corn syrup: production, uses and public health concerns.
Parker K, et al.
Biotechnology and Molecular Biology Reviews, 5, 71-78 (2010)
High-potency amyloglucosidase-producing mold of the Aspergillus niger group.
Smiley K L, et al.
Applied Microbiology, 12(5), 455-455 (1964)
Simultaneous amyloglucosidase and exo-polygalacturonase production by Aspergillus niger using solid-state fermentation.
Costa, J et al.
Brazilian Archives of Biology and Technology, 50, 759-766 (2007)
Recent Advances in Basic and Applied Aspects of Industrial Catalysis (1998)
Romy Rehschuh et al.
Plant physiology, 184(2), 852-864 (2020-08-21)
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