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PTRP12H48

Millipore

Millicell® 12 well hanging cell culture inserts

pore size 1.0 μm, clear PET membrane, diam. 12 mm, Tissue Culture (TC)-treated surface, sterile

Synonym(s):

Polyethylene Terephthalate hanging cell culture insert, cell culture inserts, permeable culture insert, plate inserts, tissue culture insert, tissue culture plate

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About This Item

UNSPSC Code:
41122107
eCl@ss:
32011202
NACRES:
NB.13

material

clear PET membrane
polystyrene housing

Quality Level

sterility

γ-irradiated
sterile

feature

2x106 pores/cm2

packaging

pack of 48 ea

manufacturer/tradename

Millicell®

parameter

37 °C max. temp.

technique(s)

cell attachment: suitable
cell culture | mammalian: suitable
cell differentiation: suitable
immunocytochemistry: suitable

H

15.5 mm

diam.

12 mm

insert maximum volume

0.8 mL

insert working volume

0.4 mL

size

12 wells

surface area

1.1 cm2

color

clear membrane
transparent membrane

pore size

1.0 μm

binding type

Tissue Culture (TC)-treated surface

shipped in

ambient

General description

Millicell® Hanging Cell Culture inserts with Polyethylene Terephthalate (PET) membrane are ideal for the growth and differentiation of various cell types. This individually sterile-packaged insert has a 1.0 μm pore size transparent membrane and fits in most 12-well plastic tissue culture plates.

In plastic tissue culture plates, cells can access media only from their apical sides, but in Millicell® tissue culture inserts, cells can access media from both their apical and basolateral sides. As result, cell growth, structure, and function more closely mimic what occurs in vivo.

The insert’s flanged design suspends it within the culture plate well and makes it possible to access both sides of the cell monolayer. Spaces between three protruding arms facilitate pipetting between the outside of the insert and the culture plate well.

Replacement for MCRP12H48

Application

  • Cell Proliferation
  • Cell Surface Receptors
  • Co-Culture
  • Epithelial Cell Growth
  • Feeder Layers
  • Fluorescent Detection / Immunohistochemistry
  • In Vitro Fertilization
  • In Vitro Toxicology
  • Microbial Attachment
  • Phase Contrast Microscopy
  • Polarized Protein Secretions
  • Polarized Uptake
  • Transport / Permeability

Packaging

Individually blister packaged

Other Notes

See user guide for recommended media volume.

Legal Information

Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

How to set up barrier formation and permeability assays using cell culture inserts and TEER measurements.

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