Gamma-1-Tubulin, also known as Gamma-tubulin complex component 1, GCP-1 or Tubulin gamma-1 chain, and encoded by the gene TUBG1/TUBG, is a form of tubulin protein and a component of microtubules. Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome and plays a major role in the organization of the microtubule cytoskeleton. Gamma-1-Tubulin is part of the pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, and it is also involved in centrosome duplication and spindle formation. Interestingly, gamma tubulin is often up regulated but improperly compartmentalized in many cancer cells including non-small cell lung cancer. Mutations in Gamma-1-Tubulin gene are associated with developmental cortical defects including microcephaly and malformations of cortical development (MCD). Gamma-1-Tubulin is localized to the cytoplasm and cytoskeleton components and expressed in a variety of cell types and tissues.
Immunogen
GST-tagged recombinant protein corresponding to human Gamma-1-Tubulin.
Application
Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected Gamma-1-Tubulin in NIH/3T3 cells.
Alexa Fluor™ is a registered trademark of Life Technologies.
This Anti-Gamma-1-Tubulin antibody, clone 6H3.1 is validated for use in western blotting & ICC for the detection of Gamma-1-Tubulin.
Quality
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 0.25 µg/mL of this antibody detected Gamma-1-Tubulin in 10 µg of HeLa cell lysate.
Target description
~56 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.
Physical form
Format: Purified
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Centrosomes and cilia are microtubule-based superstructures vital for cell division, signaling, and motility. The once thought hollow lumen of their microtubule core structures was recently found to hold a rich meshwork of microtubule inner proteins (MIPs). To address the outstanding
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