MABF3030
Anti-IL-11 Antibody, clone 12-2E-6G
Synonym(s):
Interleukin-11
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.43
Recommended Products
biological source
rat
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
12-2E-6G, monoclonal
mol wt
calculated mol wt 21.52 kDa
observed mol wt ~20 kDa
purified by
using protein G
species reactivity
mouse
packaging
antibody small pack of 100 μL
technique(s)
immunohistochemistry: suitable
western blot: suitable
isotype
IgG2aκ
epitope sequence
Unknown
Protein ID accession no.
UniProt accession no.
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
mouse ... IL11(16156)
General description
Interleukin-11 (UniProt: P47873 ; also known as IL-11) is encoded by the Il11 gene (Gene ID: 16156) in murine species. IL-11 is member of the IL-6 family of cytokines that is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off to produce mature secreted protein. It is involved in a variety of biological responses, including hematopoiesis, bone development, and carcinogenesis. Its actions are mediated via interaction with IL-11 receptor that is composed of IL-11R and gp130. Binding of IL-11 to its receptor induces the activation of JAK that phosphorylates STAT3, which then translocates to the nucleus and activates transcription of various genes. IL-11 is reported to promote the proliferation of hepatocytes in response to liver damage. In mice subjected to liver injury, IL-11 is mainly produced by hepatocytes in response to reactive oxygen species (ROS) released from dying hepatocytes. Secreted IL-11 then induces STAT3 phosphorylation in adjacent healthy hepatocytes resulting in their compensatory proliferation. It has been reported that IL-11 production in dying hepatocytes depended on ERK2 that phosphorylates Fra-1 and prevents its proteasomal degradation. Fra-1 is subsequently recruited to the Il11 promoter and activates transcription. Clone 12-2E-6G (12) is suitable for detecting endogenous IL-11 in vivo by immunohistochemistry. It is shown to detect IL-11 positive cells in colon cancer in azoxymethane plus dextran sulfate treated wild-type mice but does not detect IL-11 positive cells in Il11-deficient mice or normal colonic tissue in wild-type mice. (Ref.: Deguchi, Y., et al. (2018). Biochem. Biophys. Res. Commun. 505(2); 453-459; Nishina, T., et al. (2012). Sci. Signal. 5(207); ra5).
Specificity
Clone 12-2E-6G is a rat monoclonal antibody that detects murine IL-11.
Immunogen
FLAG-tagged full-length recombinant murine IL-11 produced in HEK293T cells transfected with pcDNA3-mIl11.
Application
Quality Control Testing
Evaluated by Western Blotting in HEK293T cells transiently transfected with murine IL-11.
Western Blotting Analysis (WB): A 1:250 dilution of this antibody detected IL-11 in HEK293T cells transiently transfected with murine IL-11, but not in wild-type HEK293T cells.
Tested Applications
Western Blotting Analysis: A representative lot detected IL-11 in Western Blotting application (Deguchi, Y., et al. (2018). Biochem Biophys Res Commun. 505(2):453-459; Nishina, T., et al. (2021). Nat Commun. 12(1):2281).
Immunohistochemistry Applications: A representative lot detected IL-11 in Immunohistochemistry application (Deguchi, Y., et al. (2018). Biochem Biophys Res Commun. 505(2):453-459; Nishina, T., et al. (2021). Nat Commun. 12(1):2281).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting in HEK293T cells transiently transfected with murine IL-11.
Western Blotting Analysis (WB): A 1:250 dilution of this antibody detected IL-11 in HEK293T cells transiently transfected with murine IL-11, but not in wild-type HEK293T cells.
Tested Applications
Western Blotting Analysis: A representative lot detected IL-11 in Western Blotting application (Deguchi, Y., et al. (2018). Biochem Biophys Res Commun. 505(2):453-459; Nishina, T., et al. (2021). Nat Commun. 12(1):2281).
Immunohistochemistry Applications: A representative lot detected IL-11 in Immunohistochemistry application (Deguchi, Y., et al. (2018). Biochem Biophys Res Commun. 505(2):453-459; Nishina, T., et al. (2021). Nat Commun. 12(1):2281).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Physical form
Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Reconstitution
0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommended storage: +2°C to +8°C.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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