The antibody reacts with human laminin both in ELISA and on cells and frozen sections. It is able to precipitate laminin from the culture medium. The antibody does not inhibit neurite outgrowth from neurons induced by laminin as well as the binding of heparin to laminin. It does not react with laminin from rat and mouse.The binding of the antibody to human laminin is not inhibited by the laminin-pentapeptide Tyr-Ile-Gly-Ser-Arg (Iwanmoto, 1987).The staining of laminin was tested on a number of materials including human lung fibroblasts (IMR 90) and human skeletal muscle tissue.
Immunogen
Pepsin fragments of human laminin from placenta.
Application
Immunocytochemistry: (5-20 μg/mL)
ELISA: (5-20 μg/mL)
Immunohistochemistry: (5-20 μg/mL) Optimal working dilutions must be determined by end user.
Research Category Cell Structure
Research Sub Category ECM Proteins
This Anti-Laminin Antibody, clone 2G6/A2 is validated for use in ELISA, IP, IC, IH for the detection of Laminin.
Physical form
Format: Purified
Purified immunoglobulin. Liquid in 0.01 M Phosphate buffer, 0.15 M NaCl, pH 7.1, sterile filtered.
Storage and Stability
Maintain at 2-8°C in undiluted aliquots for up to 12 months.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Developmental dynamics : an official publication of the American Association of Anatomists, 238(5), 1118-1130 (2009-04-23)
In routine culture, human embryonic stem (hES) cells are maintained on either feeder cells or special culture substrates such as Matrigel. However, to expand hES cells for clinical applications, it is desirable to minimize animal-derived materials in the culture for
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