MAB4709

Anti-Caspase 9 Antibody, clone 2-23

clone 2-23, Chemicon^®, from mouse

• Synonym(s): ICE-LAP6, Mch-6, Apaf-3
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 2-23, monoclonal
• species reactivity: human, mouse
• should not react with: rat
• manufacturer/tradename: Chemicon^®
• technique(s): immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG1
• NCBI accession no.: NM_001229.2; NM_032996.1
• UniProt accession no.: P55211
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... CASP9(842)

Description

Specificity

Recognizes 46-48 kDa full-length procaspase-9, human recombinant caspase-9 and procaspase-9 from human cell lysates. Highly specific to caspase-9 and shows no cross-reaction with other caspase family members. Activation of procaspase-9 by Apaf-1 in the cytochrome c pathway requires proteolytic cleavage to generate active caspase-9. Once activated, capsase-9 initiates a caspase cascade involving the downstream executioner caspases 3, 6 and 7.

Immunogen

Recombinant human caspase-9 prodomain

Application

Anti-Caspase 9 Antibody, clone 2-23 is an antibody against Caspase 9 for use in IP, WB, IH(P).

Research Category
Apoptosis & Cancer

Metabolism

Research Sub Category
Caspases

Enzymes & Biochemistry

Western blot: 1:1000 Immunohistochemistry (frozen and paraffin): 1:500 - 1:1000

Optimal working dilutions must be determined by end user.

Linkage

Replaces: 04-443

Physical form

Format: Purified

Purified immunoglobulin by protein A. Liquid in PBS, pH 7.6, with 0.1% sodium azide.

Storage and Stability

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable