HMYOMAG-56K

MILLIPLEX^® Human Myokine Magnetic Bead Panel

The Human Myokine Panel, using the Luminex xMAP technology, enables the simultaneous analysis of 15 myokine protein biomarkers in human serum, plasma and cell/tissue culture samples.

• UNSPSC Code: 12161503
• eCl@ss: 32161000
• NACRES: NA.84

Properties

• Quality Level: 200
• species reactivity: human
• manufacturer/tradename: Milliplex^®
• assay range: accuracy: 100%; (Apelin); accuracy: 92%; (IL-15); accuracy: 96%; (FSTL-1); accuracy: 97%; (LIF); accuracy: 98%; (Oncostatin M (OSM)); sensitivity: 0.9 pg/mL; (MinDC+2SD; IL-6); sensitivity: 14 pg/mL; (MinDC+2SD; FGF-21); sensitivity: 259 pg/mL; (MinDC+2SD; Myostatin/GDF8); sensitivity: 281 pg/mL; (MinDC+2SD; Irisin); sensitivity: 4 pg/mL; (MinDC+2SD; LIF); sensitivity: 40 pg/mL; (MinDC+2SD; Fractalkine/CX3CL1); sensitivity: 5 pg/mL; (MinDC+2SD; IL-15); sensitivity: 59 pg/mL; (MinDC+2SD; Osteocrin/Musclin); sensitivity: 6 pg/mL; (MinDC+2SD; Oncostatin M (OSM)); sensitivity: 64 pg/mL; (MinDC+2SD; Apelin); sensitivity: 7.5 ng/mL; (MinDC+2SD; Osteonectin (SPARC)); sensitivity: 737 pg/mL; (MinDC+2SD; Follistatin-like Protein 1 (FSTL1)); sensitivity: 815 pg/mL; (MinDC+2SD; Erythropoietin (EPO)); sensitivity: 9 pg/mL; (MinDC+2SD; BDNF); sensitivity: 9.8 pg/mL; (MinDC+2SD; FABP3); standard curve range: 0.6-2,400 pg/mL; (IL-6); standard curve range: 10-40,000 pg/mL; (LIF); standard curve range: 122-500,000 pg/mL; (Apelin); standard curve range: 2-10,000 pg/mL; (BDNF); standard curve range: 2-10,000 pg/mL; (IL-15); standard curve range: 2-6,000 pg/mL; (Oncostatin M (OSM)); standard curve range: 24-100,000 pg/mL; (FABP3); standard curve range: 244-1,000,000 pg/mL; (Erythropoietin (EPO)); standard curve range: 244-1,000,000 pg/mL; (Irisin); standard curve range: 488-2,000,000 pg/mL; (Follistatin-like Protein 1 (FSTL1)); standard curve range: 488-2,000,000 pg/mL; (Myostatin/GDF8); standard curve range: 49-200,000 pg/mL; (Fractalkine/CX3CL1); standard curve range: 49-200,000 pg/mL; (Osteocrin/Musclin); standard curve range: 5-20,000 pg/mL; (FGF-21); standard curve range: 7-30,000 ng/mL; (Osteonectin (SPARC)); inter-assay cv: <15%; intra-assay cv: <10%; (Apelin); inter-assay cv: <15%; intra-assay cv: <10%; (BDNF); inter-assay cv: <15%; intra-assay cv: <10%; (Erythropoietin (EPO)); inter-assay cv: <15%; intra-assay cv: <10%; (FABP3); inter-assay cv: <15%; intra-assay cv: <10%; (FGF-21); inter-assay cv: <15%; intra-assay cv: <10%; (Follistatin-like Protein 1 (FSTL1)); inter-assay cv: <15%; intra-assay cv: <10%; (Fractalkine/CX3CL1); inter-assay cv: <15%; intra-assay cv: <10%; (IL-15); inter-assay cv: <15%; intra-assay cv: <10%; (IL-6); inter-assay cv: <15%; intra-assay cv: <10%; (Irisin); inter-assay cv: <15%; intra-assay cv: <10%; (LIF); inter-assay cv: <15%; intra-assay cv: <10%; (Myostatin/GDF8); inter-assay cv: <15%; intra-assay cv: <10%; (Oncostatin M (OSM)); inter-assay cv: <15%; intra-assay cv: <10%; (Osteocrin/Musclin); inter-assay cv: <20%; intra-assay cv: <10%; (Osteonectin (SPARC))
• technique(s): multiplexing: suitable
• detection method: fluorometric (Luminex xMAP)
• shipped in: wet ice

Description

General description

Muscle contraction during physical activity is an important activator of the release of the myokines. Myokines not only act in an autocrine and/or paracrine manner in regulation of skeletal muscle growth, but they also act as endocrine hormones to mediate inter-organ crosstalk. The myokine theory provides molecular mechanistic explanations for exercise-induced metabolic changes in liver and adipose tissue and for the profound changes found in immune and neuronal systems.

MILLIPLEX^® Human Myokine Panel is a15-plex kit to be used for the simultaneous quantification of any or all of the following analytes in human serum,plasma and cell/tissue supernatant samples: Fractalkine/CX3CL1, BDNF, Oncostatin M (OSM), Osteonectin (SPARC), LIF, FGF-21, Erythropoietin (EPO), IL-15, IL-6, Apelin, Myostatin/GDF8, Irisin, Osteocrin/Musclin, FABP3 and Follistatin-Like Protein (FSTL 1). This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Metabolism

Application

• Analytes: Apelin (APLN), Brain-derived Neurotrophic Factor (BDNF), Erythropoeitin (EPO), Fatty Acid-Binding Protein 3 (FABP3), Fibroblast Growth Factor 21 (FGF21), Fractalkine (CX3CL1), Follistatin-Like 1 Protein (FSTL-1), IL-6, IL-15, Irisin, Leukemia Inhibitory Factor (LIF), Myostatin (MSTN/GDF8), Oncostatin M (OSM), Osteocrin (OSTN/Musclin), Osteonectin (SPARC)
• Recommended Sample Type: Human serum, plasma, and cell/tissue culture supernatants or lysates
• NOTE: Substantial amounts of BDNF are stored in circulating platelets and subsequently released upon platelet activation. Therefore, platelet-poor plasma is critical to ensure accurate measurement of circulating levels of BDNF.
• Recommended Sample Dilution: 25 μL per well of 1:2 diluted serum or plasma. Cell/tissue culture samples may be run neat or appropriately diluted in control media.
• Assay Run Time: Overnight (16-18 hours) at 2-8°C or 2 hours at room temperature (20-25°C)
• Research Category: Bone
• Research Subcategory: Metabolism

Features and Benefits

Design your multiplex kit by choosing available analytes within this panel.

Other Notes

Please contact Technical Service for linearity of dilution.

Legal Information

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

Safety Information

• Pictograms: GHS06,GHS08,GHS05,GHS09
• Signal Word: Danger
• Hazard Statements: H302 + H332,H311,H315,H317,H318,H373,H411
• Precautionary Statements: P273 - P280 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338 - P314
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2
• Target Organs: Respiratory Tract
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects