AB19016
Anti-MMP-9 Antibody, Catalytic domain
Chemicon®, from rabbit
Synonym(s):
Gelatinase B, 92 kDa Type IV Collagenase
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human, mouse, rat
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... MMP9(4318)
General description
All cells within tissues are surrounded by an extracellular matrix (ECM) giving the tissues shape and structure. The ECM is constantly being remodeled and constant communication is maintained between cells through this matrix. Secreted proteins, termed matrix metalloproteinases (MMPs), are involved in the modulation of cell matrix interactions. MMPs are Zn(2+) binding endopeptidases that degrade various components of the ECM.
MMPs are enzymes implicated in normal and pathologic tissue remodeling processes, wound healing, angiogenesis, and tumor invasion. MMP9, also known as gelatinase B, is a secreted enzyme which degrades the interstitial collagens, types I, II, and III, and is produced by normal alveolar macrophages and granulocytes. The expression of MMP9 increases in Epstein-Barr virus infected lymphoma derived cell lines and may be of significance in typically invasive nasopharyngeal carcinomas.
MMPs are enzymes implicated in normal and pathologic tissue remodeling processes, wound healing, angiogenesis, and tumor invasion. MMP9, also known as gelatinase B, is a secreted enzyme which degrades the interstitial collagens, types I, II, and III, and is produced by normal alveolar macrophages and granulocytes. The expression of MMP9 increases in Epstein-Barr virus infected lymphoma derived cell lines and may be of significance in typically invasive nasopharyngeal carcinomas.
Specificity
Reactivity with other species has not been confirmed.
Recognizes rat MMP-9 pro and active forms. Antibody also reacts with mouse MMP-9 and human MMP-9, other species not tested. Exhibits no cross-reactivity with other MMP family members. The antibody was generated using E. coli-expressed active rat 92 kDa type IV collagenase (catalytic domain) as an immunogen.
Immunogen
E. coli-expressed active rat 92 kDa type IV collagenase (catalytic domain)
Epitope: Catalytic domain
Application
Anti-MMP-9 Antibody, Catalytic domain is an antibody against MMP-9 for use in IH, IP & WB.
Research Category
Cell Structure
Cell Structure
Research Sub Category
MMPs & TIMPs
MMPs & TIMPs
Western Blot Analysis:
1:1000 dilution of a previous lot was used.
Immunoprecipitation:
1:500 dilution from a previous lot was used. MMP-9 is constitutively produced by some tumor cell lines (i.e., HT1080, HL60, and U937), but not in most quiescent cells and tissues. The phorbol ester TPA stimulates production of MMP-9 in some cell types. MMP-9 levels in culture media (pg/mL) are often below the threshold of detection by standard Western Blotting. The enzyme can be concentrated from culture media by gelatin-agarose affinity chromatography (Goldberg, G.I. et al. J. Biol. Chem. 267, 4583-4591. 1992). This can prevent appearance of a spurious band of antibody binding in the vicinity of 68 kDa displayed by some concentrated media.
Western blot: 1:2,000. Typically 98-95 kDa pro and 92-88kDa active forms are detected as well as breakdown products under fully reduced conditions. Under non-reduced conditions additional bands may be detected because of SDS-stable TIMP and other inhibitor complexes. CC069 is recommended as a positive control.
Immunohistochemistry: 1:100 dilution from a previous lot was used in frozen acetone fixed tissues.
Optimal working dilutions must be determined by end user.
1:1000 dilution of a previous lot was used.
Immunoprecipitation:
1:500 dilution from a previous lot was used. MMP-9 is constitutively produced by some tumor cell lines (i.e., HT1080, HL60, and U937), but not in most quiescent cells and tissues. The phorbol ester TPA stimulates production of MMP-9 in some cell types. MMP-9 levels in culture media (pg/mL) are often below the threshold of detection by standard Western Blotting. The enzyme can be concentrated from culture media by gelatin-agarose affinity chromatography (Goldberg, G.I. et al. J. Biol. Chem. 267, 4583-4591. 1992). This can prevent appearance of a spurious band of antibody binding in the vicinity of 68 kDa displayed by some concentrated media.
Western blot: 1:2,000. Typically 98-95 kDa pro and 92-88kDa active forms are detected as well as breakdown products under fully reduced conditions. Under non-reduced conditions additional bands may be detected because of SDS-stable TIMP and other inhibitor complexes. CC069 is recommended as a positive control.
Immunohistochemistry: 1:100 dilution from a previous lot was used in frozen acetone fixed tissues.
Optimal working dilutions must be determined by end user.
Quality
Routinely evaluated by Western Blot on L6 lysates.
Western Blot Analysis: 1:1000 dilution of this lot detected MMP-9 on 10 µg of L6 lysates.
Western Blot Analysis: 1:1000 dilution of this lot detected MMP-9 on 10 µg of L6 lysates.
Target description
92 kDa
Linkage
Replaces: 04-1150
Physical form
Format: Purified
Protein A purified
Purified rabbit polyclonal in buffer containing PBS, containing 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HL60 cell lysate, U937 cell lysate Lung.
HL60 cell lysate, U937 cell lysate Lung.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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