251530-M

Dafadine-A

• Synonym(s): Dafadine-A, DAF-9 Cytochrome P450 Inhibitor, Dafadine-A, (5-((2,6-Dimethylphenoxy)methyl)isoxazol-3-yl)(4-(pyridin-4-yl)piperidin-1-yl)methanone, 4-(1-((5-((2,6-Dimethylphenoxy)methyl)-3-isoxazolyl)carbonyl)-4-piperidinyl)pyridine
• Empirical Formula (Hill Notation): C₂₃H₂₅N₃O₃
• Molecular Weight: 391.46
• UNSPSC Code: 12352200

Properties

• Assay: ≥98% (HPLC)
• form: powder
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: light beige
• solubility: DMSO: 100 mg/mL
• storage temp.: 2-8°C

Description

General description

A cell-permeable isoxazoloamide compound that specifically inhibits DAF-9 activity in C. elegans (~20 µM) without affecting the activity of DAF-12 and sterol- and oxysterol-metabolizing P450s. Reversibly targets the catalytic heme iron of DAF-9 cytochrome p450 and induce a constitutive dauer (Daf-c), distal-tip cell migration (Mig) and protruding vulval (Pvl) phenotypes in wild-type C.elegans (at ~25 µM). Also, shown to be one of the most potent inhibitors of CYP27A1, the mammalian ortholog of DAF-9. Robustly promotes dauer formation (non-aging stress-resistant alternative third larval stage) only in wild type C. elegans and extends their life span by about 29%.

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Luciani, G.M., et al. 2011. Nat. Chem. Biol.7, 891.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): 545.7 - 659.1 °F
• Flash Point(C): 285.4 - 348.4 °C