Anti-Aquaporin 5, rabbit polyclonal, recognizes the ~28 kDa AQP 5 protein in epithelial tissue extracts. It is validated for ELISA, Western blotting, immunofluorescence, and immunohistochemistry.
Recognizes the ~28 kDa AQP 5 protein in epithelial tissue extracts.
Immunogen
a synthetic peptide corresponding to a 17-amino acid sequence in the cytoplasmic region of rat AQP5
Application
ELISA (0.5-1.0 µg/ml)
Immunoblotting (1-10 µg/ml)
Immunofluorescence (2-20 µg/ml)
Immunohistochemistry (2-20 µg/ml)
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Analysis Note
Positive Control Epithelial cells
Other Notes
Does not cross-react with other AQP proteins. The use of 0.5-1.0% milk in all primary/secondary antibody-enzyme conjugate incubations is recommended in order to suppress non-specific bands. Variables associated with assay conditions will dictate the optimal working dilution.
Lee, M.D., et al. 1996. J. Biol. Chem. 271, 8599. Raina, S., et al. 1995. J. Biol. Chem. 270, 1908.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 52(10), 1341-1350 (2004-09-24)
Corneas with edema-related diseases lose transparency, which causes significant vision loss. This study analyzed seven aquaporins (AQPs) in normal corneas, pseudophakic/aphakic bullous keratopathy (PBK/ABK) corneas, Fuchs' dystrophy corneas, keratoconus corneas, post-cataract surgery (PCS) corneas, and normal organ-cultured corneas. RNA levels
The Journal of clinical investigation, 119(2), 336-348 (2009-01-24)
The bone marrow compartment is enriched in stem and progenitor cells, and an unidentified subpopulation of these cells can contribute to lung epithelial repair. Here we identify this subpopulation and quantitate its relative contribution to injured airway epithelium. A subpopulation
Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology, 39(3), 267-273 (2021-05-28)
This study aims to investigate the effects of ionizing radiation on the secretion of the paracellular pathway in rat submandibular glands (SMGs) and reveal the changes in the tight junction (TJ) protein claudin-4. A total of 24 Wistar rats were
A rare population of salivary gland cells isolated based on c-Kit immunoreactivity are thought to represent tissue stem cells since they exhibit the most robust proliferative and differentiation capacity ex vivo. Despite their high promise for cell-based therapies aimed at
Aquaporins (AQPs) are essential to coordinate the transit of water and ions through the cell membrane. In salivary glands (SGs), AQPs have been associated with saliva formation, facilitating water absorption through the epithelium during the formation of hypotonic saliva, which
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