07-1417
Anti-SGLT-1 (Sodium Glucose Co-transporter-1) Antibody
serum, from rabbit
Synonym(s):
Sodium/glucose cotransporter 1, Solute carrier family 5 member 1, Solute carrier family 5 (sodium/glucose cotransporter), member 1, Solute carrier family 5 (sodium/glucose transporter),member 1
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All Photos(4)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
pig, rat, avian, rabbit, mouse, canine, human, reptile, feline
technique(s)
ELISA: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... SLC5A1(6523)
General description
The kidneys play a major role in the regulation of blood glucose. Although kidney nephrons indiscriminatly filter about 180g of glucose per day from the blood, most is reabsorbed actively in the proximal convoluted tubule by a Na+/Gl co-transport (ratio 2:1) mechanism (SGLT). Typically, glucose is first reabsorbed by these transporters, which reside within the brush-border epithelia of the PCT cells. The glucose is then transported out of the cell across the basolateral membranes by a facilitated diffusion sugar transporter (GLUTs). At least three members of SGLT′s (SGLT1-3) have been cloned and characterized from various species. SGLT1-3 genes code for protein of approximately 659-672 residues, with both N and C termini predicted to be extracellular. There is approximately 60-70% homology between the 3 forms. SGLT1 does not discriminate a-MDG, glucose and galactose, while SGLT2 and 3 do not transport D-galactose efficiently. SGLT1 is an integral membrane protein expressed in proximal convoluted tubule Sq1 segments of the kidney (outer renal medulla) and in the intestine.
Specificity
Recognizes SGLT-1.
Immunogen
Synthetic peptide corresponding to amino acids 402-420 in the cytoplasmic region of SGLT-1 of mouse/rabbit small intestine.
Application
Anti-SGLT-1 (Sodium Glucose Co-transporter-1) Antibody detects level of SGLT-1 (Sodium Glucose Co-transporter-1) & has been published & validated for use in WB, IH(P), ELISA.
Immunohistochemisty (paraffin): Anti-SGLT-1 staining on human kidney, tissue pretreated with citrate buffer, pH 6.0. A previous lot of this antibody was used at 1 μg/mL, using IHC-Select detection with HRP-DAB. Immunoreactivity is detected in the brush boarder of proximal convoluted tubule.
ELISA: 1:10,000 - 1:50,000 dilution of a previous lot, using 50-100 ng of control peptide/well.
Optimal working dilutions must be determined by end user.
Western Blot: A previous lot of this antibody was used on multiple lysates. See datasheet.
ELISA: 1:10,000 - 1:50,000 dilution of a previous lot, using 50-100 ng of control peptide/well.
Optimal working dilutions must be determined by end user.
Western Blot: A previous lot of this antibody was used on multiple lysates. See datasheet.
Research Category
Signaling
Signaling
Research Sub Category
Insulin/Energy Signaling
Insulin/Energy Signaling
Quality
Routinely evaluated by Western Blot on PC3 lysates.
Western Blot Analysis:
1:500-1:2,000 dilution of this lot detected SGLT-1 on 10 μg of PC3 lysates.
Western Blot Analysis:
1:500-1:2,000 dilution of this lot detected SGLT-1 on 10 μg of PC3 lysates.
Target description
Recognizes a band of approximately 70-77 kDa in Western blot.
Linkage
Replaces: AB1352
Physical form
Rabbit polyclonal IgG antiserum in buffer containing 0.1% sodium azide.
Storage and Stability
Stable for 1 year at -20°C from date of receipt in undiluted aliquots.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
PC3 cell lysate
PC3 cell lysate
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Identification of two unique polypeptides from dog kidney outer cortex and outer medulla that exhibit different Na+/D-glucose cotransport functional properties.
Silverman, M, et al.
Biochimica et Biophysica Acta, 1153, 43-52 (1993)
Molecular biology approaches to comparative study of Na(+)-glucose cotransport.
Pajor, A M, et al.
The American Journal of Physiology, 263, R489-R495 (1992)
Balasubramanian Palaniappan et al.
Nutrients, 12(10) (2020-10-18)
In mammalian small intestine, glucose is primarily absorbed via Na-dependent glucose co-transporter (SGLT1) on the brush border membrane (BBM) of absorptive villus cells. Malabsorption of nutrients (e.g., glucose) leads to malnutrition, a common symptom of inflammatory bowel disease (IBD), where
Léia Cardoso-Sousa et al.
PloS one, 14(9), e0222575-e0222575 (2019-09-20)
Respiratory infection can be exacerbated by the high glucose concentration in the airway surface liquid (ASL). We investigated the effects of salbutamol and phlorizin on the pulmonary function, oxidative stress levels and SGLT1 activity in lung, pulmonary histopathological damages and
Michael S Balzer et al.
Biomolecules, 10(11) (2020-11-25)
Peritoneal dialysis (PD) is limited by glucose-mediated peritoneal membrane (PM) fibrosis, angiogenesis, and ultrafiltration failure. Influencing PM integrity by pharmacologically targeting sodium-dependent glucose transporter (SGLT)-mediated glucose uptake has not been studied. In this study, wildtype C57Bl/6N mice were treated with
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