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Merck

Application of the Fenton process in the elimination of helminth eggs.

Journal of water and health (2014-12-05)
Sandra Isabel Escobar-Megchún, Hugo Alejandro Nájera-Aguilar, Magaly González-Hilerio, Javier Gutiérrez-Jiménez, Rubén Fernando Gutiérrez-Hernández, María Neftalí Rojas-Valencia
ABSTRACT

This study relates to a method for evaluating the degradation efficiency of Ascaris lumbricoides eggs through a Fenton reaction, using hydrogen peroxide (H2O2) at 100, 250, and 500 mg/L, ferrous sulfate (FeSO4) at 458 mg/L Fe(2+) and pH values of 3, 4.5, and 6. The experiments were conducted according to a 3(2) experimental design, with 1:1, 3:1, and 5:1 (H2O2/Fe(2+)) molar ratios. The oxidation and flocculation stages were performed at 130 rpm during 2 hours and at 25-30 rpm during 20 min, respectively. As a result of the Fenton reaction, an average of 91.2% Ascaris lumbricoides egg degradation was achieved at pH 6 using a 500 mg/L dose of H2O2 and a 3:1 (H2O2/Fe(2+)) molar ratio. Thus, this process is an alternative for eliminating parasites that are resistant to conventional disinfection processes and significantly reduces the human health hazard they represent.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Hydrogen peroxide solution, tested according to Ph. Eur.
Millipore
Hydrogen peroxide solution, 3%, suitable for microbiology
Sigma-Aldrich
Iron, puriss. p.a., carbonyl-Iron powder, low in magnesium and manganese compounds, ≥99.5% (RT)
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Hydrogen peroxide solution, 34.5-36.5%
Iron, IRMM®, certified reference material, 0.5 mm wire
Sigma-Aldrich
Iron, foil, thickness 0.1 mm, ≥99.9% trace metals basis
Sigma-Aldrich
Iron, powder, −325 mesh, 97%
Sigma-Aldrich
Iron, chips, 99.98% trace metals basis
Supelco
Hydrogen peroxide solution, ≥30%, for trace analysis
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Iron(II) sulfate heptahydrate, suitable for plant cell culture, ≥99%
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Iron(II) sulfate heptahydrate, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99%
Sigma-Aldrich
Hydrogen peroxide solution, 30 % (w/w) in H2O, contains stabilizer
Supelco
Hydrogen peroxide solution, 30 % (w/w), for ultratrace analysis
Sigma-Aldrich
Iron(II) sulfate heptahydrate, ACS reagent, ≥99.0%
Iron, wire reel, 5m, diameter 0.025mm, as drawn, 99.99+%
Iron, wire reel, 1m, diameter 0.5mm, as drawn, 99.998%
Iron, wire reel, 0.5m, diameter 0.25mm, hard, 99.99+%
Iron, wire reel, 0.5m, diameter 1.0mm, as drawn, 99.99+%
Iron, wire reel, 5m, diameter 0.25mm, hard, 99.99+%
Iron, wire reel, 50m, diameter 0.5mm, as drawn, 99.95%
Iron, wire reel, 2m, diameter 0.25mm, annealed, 99.99+%
Iron, wire reel, 0.05m, diameter 0.5mm, as drawn, 99.998%
Iron, rod, 500mm, diameter 12.7mm, as drawn, 99.95%
Iron, foil, 50x50mm, thickness 2mm, as rolled, 99.99+%
Iron, tube, 200mm, outside diameter 4.0mm, inside diameter 3.6mm, wall thickness 0.2mm, as drawn, 99.5%
Iron, rod, 200mm, diameter 25mm, as drawn, 98+%
Iron, tube, 100mm, outside diameter 8.0mm, inside diameter 5mm, wall thickness 1.5mm, annealed, 99.5%
Iron, foil, 300x300mm, thickness 1.0mm, as rolled, 99.5%
Iron, foil, 300x300mm, thickness 2.0mm, as rolled, armco« soft ingot 99.8%
Iron, wire reel, 1m, diameter 0.025mm, as drawn, 99.99+%