G006022
HPLC Analysis of Peptides from BSA on Proteomix® SCX-NP3, Volatile Buffer
application for HPLC
About This Item
technique(s)
HPLC: suitable
test parameters
column: Proteomix SCX-NP1.7, 3 cm x 2.1 mm I.D., 1.7 μm particles (Z777142)
mobile phase: (A) 10 mM sodium phosphate, pH 2.5; (B) A + 1 M NaCl; (C) acetonitrile
gradient: 90% A, 0% B, 10% C held for 1.5 min; to 40% A, 50% B, 10% C in 18.5 min
pressure: 5511 psi (380 bar)
detector: UV, 214 nm
sample: trypsin digested bovine serum albumin (BSA)
flow rate: 0.35 mL/min
suitability
application for HPLC
application(s)
life science and biopharma
Legal Information
analytical column
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