W1754
Water
PCR Reagent, suitable for PCR
Synonym(s):
H2O
About This Item
Recommended Products
product name
Water, PCR Reagent
grade
PCR Reagent
vapor density
<1 (vs air)
vapor pressure
3 mmHg
sterility
sterile-filtered
form
liquid
packaging
vial of 1.5 mL
technique(s)
PCR: suitable
refractive index
n20/D 1.34 (lit.)
pH
5-7
bp
100 °C (lit.)
mp
0 °C (lit.)
density
1.000 g/mL at 3.98 °C (lit.)
foreign activity
DNase, none detected
RNase, none detected
SMILES string
O
InChI
1S/H2O/h1H2
InChI key
XLYOFNOQVPJJNP-UHFFFAOYSA-N
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General description
Application
- as a component of the reaction mixture and as a diluting agent in microfluidic RT-qPCR
- as a component of the reaction mixture for the amplification of products from fungal (Trametes versicolor) DNA
- as a diluting agent and as a component of the reaction mixture for the amplification of cDNA
- Water has been used to make up the final volume of the sample in polymerase chain reaction (PCR)
Suitability
Other Notes
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
Certificates of Analysis (COA)
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Protocols
Hot start dNTP protocol enhances specificity in PCR by blocking DNA polymerase nucleotide incorporation during PCR.
Protocol describes amplification of DNA through quantitative PCR with SYBR Green. Consistent batch-to-batch performance can be achieved with large numbers of PCR reactions.
Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures. AccuTaq LA.
Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.
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