This method utilizes sodium dodecylsulfate, included in the Lowry reagent, to facilitate the dissolution of relatively insoluble lipoproteins. An optional deoxycholate-TCA precipitation step permits removal of interfering substances such as Tris, sucrose, EDTA, salts, etc.
Application
Total Protein Kit, Micro Lowry, Peterson′s Modification has been used to measure protein content.[1][2][3]
Features and Benefits
Complete kit for protein assays
Most widely cited protein assay
Less protein to protein variation than other assays
Contains reagents that can remove interfering substances
Principle
An optional deoxycholate-TCA precipitation step permits removal of interfering substances such as Tris, sucrose, EDTA, salts, etc. Absorbance at 500-800 nm (typically read at 750 nm) is used to determine protein concentration from a standard curve.
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Products for traditional and alternative protein quantitation techniques available, including BCA, Bradford, Lowry, and more.
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