SML3673

Lin28 inhibitor C1632

≥98% (HPLC)

• Synonym(s): Lin28 1632, Lin28 inhibitor I, Lin28-C1632, Lin28-let-7 antagonist 1, N-Methyl-N-[3-(3-methyl[1,2,4]triazolo[4,3-b]pyridazin-6-yl)phenyl]acetamide
• Empirical Formula (Hill Notation): C₁₅H₁₅N₅O
• CAS Number: 108825-65-6
• Molecular Weight: 281.31
• UNSPSC Code: 12352200
• NACRES: NA.28
• MDL number: MFCD01567008
• Assay: ≥98% (HPLC)
• Form: powder

Properties

• Quality Segment: 100
• assay: ≥98% (HPLC)
• form: powder
• color: , White to light Brown
• solubility: DMSO: 2 mg/mL, clear
• storage temp.: 2-8°C
• SMILES string: [n]21nc(ccc2nnc1C)c3cc(ccc3)N(C)C(=O)C
• InChI: 1S/C15H15N5O/c1-10-16-17-15-8-7-14(18-20(10)15)12-5-4-6-13(9-12)19(3)11(2)21/h4-9H,1-3H3
• InChI key: WPAQLESUVYGUJZ-UHFFFAOYSA-N

Description

Biochem/physiol Actions

Compound 1632 (C1632) is a RNA-binding protein Lin28 inhibitor that prevents Lin28-mediated inhibition of let-7 microRNA (miRNA) maturation by blocking Lin28 interaction with let-7 miRNA precursor (IC50 = 8 μM by ELISA using truncated pre-let-7a-2). C1632 specifically upregulates Huh7 cellular let-7a/g/f miRNA, but not RNU44 snoRNA (60 μM for 48h), inhibits the stemness and induces differentiation of mouse embryonic stem cells (mESCs, 20 μM for 48h), as well as inhibits the proliferation and stem-like properties of human cancer stem cells (CSCs) in 22Rv1 and Huh7 cultures (IC50 20-42 μM).

RNA-binding protein Lin28 inhibitor that prevents Lin28-mediated inhibition of let-7 microRNA (miRNA) maturation.

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable