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SAB4200685

Sigma-Aldrich

Anti-Glucagon antibody, Mouse monoclonal

clone K79bB10, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-Glucagon antibody produced in mouse, GCG, GLP1, GLP2, GRPP

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About This Item

UNSPSC Code:
51111800
NACRES:
NA.41

biological source

mouse

Quality Level

200

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

K79bB10, monoclonal

form

buffered aqueous solution

species reactivity

cat, rabbit, mouse, guinea pig, rat, dog, porcine, zebrafish, human

packaging

antibody small pack of 25 μL

concentration

~1 mg/mL

technique(s)

immunohistochemistry: 5-10 μg/mL using heat−retrieved formalin-fixed, paraffin-embedded Rat pancreas sections and Biotin/ExtrAvidin®-Peroxidase staining system.
radioimmunoassay: suitable

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

cat ... Gcg(101097825)
dog ... Gcg(403571)
guinea pig ... Gcg(100135526)
human ... GCG(2641)
mouse ... Gcg(14526)
pig ... Gcg(397595)
rabbit ... Gcg(100341777)
rat ... Gcg(24952)

General description

Glucagon is a hormone peptide produced in Langerhans islets α-cells in the pancreas. The active form of glucagon is derived from the cleavage of preproglucagon (GCG). Additional cleavage peptides are oxyntomodulin and glucagon-like peptide-1 (GLP1).

Immunogen

Polymerized porcine glucagon

Application

Monoclonal Anti-Glucagon antibody produced in mouse has been used in immunofluorescence staining. The product may be used in several immunochemical techniques including immunohistochemistry and radioimmunoassay (RIA).

Biochem/physiol Actions

The biological activities of pancreatic glucagon include glycogenolysis, lipolysis, gluconeogenesis and ketogenesis. Glucagon has an antagonistic effect in the maintenance of normoglycemia to those of insulin and is suppressed by GLP1. Thus up-regulation of glucagon is leading to increased blood glucose levels. Glucagon-specific antibodies would prove useful as a Langerhans islets α cell, glucagonomas and tumor markers applying immunohistochemical techniques, and as an analytical tool in quantification of the hormone.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Legal Information

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Pancreatic regulation of glucose homeostasis
Roder PV, et al.
Experimental & Molecular Medicine, 48(3), e219-e219 (2016)
Metabolic regulation of GLP-1 and PC1/3 in pancreatic
Sancho V, et al.
Testing, 12 (2017)
Qingsheng Liu et al.
Advanced materials (Deerfield Beach, Fla.), 33(39), e2102852-e2102852 (2021-08-08)
Encapsulation of insulin-producing cells is a promising strategy for treatment of type 1 diabetes. However, engineering an encapsulation device that is both safe (i.e., no cell escape and no breakage) and functional (i.e., low foreign-body response (FBR) and high mass
The role of glucagon on type 2 diabetes at a glance
Godoy-Matos AF, et al.
Diabetology & Metabolic Syndrome, 6 (2014)
Abhishek A Kulkarni et al.
Oxidative medicine and cellular longevity, 2018, 1324739-1324739 (2018-05-23)
It is well known that a chronic state of elevated reactive oxygen species (ROS) in pancreatic β-cells impairs their ability to release insulin in response to elevated plasma glucose. Moreover, at its extreme, unmitigated ROS drives regulated cell death. This
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