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S1797

Sigma-Aldrich

SOC Medium

For use in transformation

Synonym(s):

Super Optimal broth

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10 X 5 ML
€225.00
100 ML
€414.00

€225.00

Estimated to ship on20 March 2025

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10 X 5 ML
€225.00
100 ML
€414.00

About This Item

UNSPSC Code:
41106200
NACRES:
NA.85

€225.00

Estimated to ship on20 March 2025

grade

for molecular biology

Quality Level

500

sterility

0.2 μm filtered

form

liquid

composition

Dextrose, 3.603 g/L
KCl, 0.186 g/L
MgSO4, 4.8 g/L
Tryptone, 20 g/L
Yeast extract, 5 g/L

technique(s)

microbiological culture: suitable

application(s)

agriculture

storage temp.

2-8°C

suitability

nonselective for Escherichia coli
nonselective for coliforms

Related Categories

General description

Super optimal medium with catabolic repressor (SOC) medium is a glucose-rich microbial growth medium used primarily in the recovery step of Escherichia coli competent cell transformations. The culture of E.coli cells in this nutrient-rich microbial broth increases the transformation efficiency of recombinant plasmids containing toxic protein genes by inhibiting the unintended activation of the lac promoter carried on the cloning vector.[1] SOC medium contains peptides, amino acids, water-soluble vitamins, and glucose in a low-salt formulation.

Application

SOC Medium has been used for the transformation of DH5α competent E-coli cells. [2] It has also been used to culture white colonies post-cloning for the identification of the recombinant protein of interest. [3]

Features and Benefits

SOC liquid provides:
  • Filter-sterilized, ready-to-use format
  • Convenient package sizes
  • Standard formulation

related product

Product No.
Description
Pricing

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
SLCN8706
SLCL9800
SLCN2539
SLCF7416
SLCG7766

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Rebekah M Martin et al.
mSystems, 3(3) (2018-07-03)
Despite insights gained through experimental models, the set of bacterial genes important for human infection is unclear for many of our most threatening pathogens. Klebsiella pneumoniae is a leading cause of health care-associated infections (HAIs) and commonly colonizes hospitalized patients
Daniel C Volke et al.
Bio-protocol, 11(4), e3917-e3917 (2021-03-19)
Precise genome engineering has become a commonplace technique for metabolic engineering. Also, insertion, deletion and alteration of genes and other functional DNA sequences are essential for understanding and engineering cells. Several techniques have been developed to this end (e.g., CRISPR/Cas-assisted
Detection of Cryptosporidium parvum Oocysts on Fresh Produce Using DNA Aptamers
Iqbal A
PLoS ONE (2015)
S Zhao et al.
Applied and environmental microbiology, 75(24), 7624-7630 (2009-10-27)
Ampicillin-resistant (Amp(r)) Salmonella enterica isolates (n = 344) representing 32 serotypes isolated from retail meats from 2002 to 2006 were tested for susceptibility to 21 other antimicrobial agents and screened for the presence of five beta-lactamase gene families (bla(CMY), bla(TEM)
Qiao-Yang Sun et al.
Analytical biochemistry, 394(1), 144-146 (2009-07-23)
In this paper, we report a useful protocol for cloning toxic protein genes. Use of the SOC medium, which is a glucose-containing rich medium, significantly improved the transformation efficiency of a recombinant plasmid containing a toxic plant subtilase SaSBT1 cDNA.
View All Related Papers

Articles

Bacterial Transformation Protocols

General protocols for growth of competent cells and their transformation (uptake of DNA).

Protocols

Competent Cell Protocols

Technical Article on competent cells. Transformation is a process by which some bacteria take up foreign genetic material (naked DNA) from the environment.

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

Questions

1–4 of 4 Questions  

  1. That is the shelf life of SOC medium if kept refrigerated at 4C?

    1 answer

    1. This product is not assigned an expiration date. It is assigned a recommended retest date of approximately 2 years after the quality release date. A recommended retest date is the time during which the product is expected to remain within established stability specifications, provided that it has been stored under defined conditions. After the Retest Date, product samples should be examined to ensure that the product is still in compliance with the established specifications.

      For more information, you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/US/en/life-science/quality-and-regulatory-management/quality-services.

      Helpful?

  2. Why is SOC Medium (S1797) used to recover bacterial cells before transformation?

    1 answer

    1. SOC is a rich bacterial broth that also contains glucose, which aids in the recovery process and prepares the cells for efficient transformation.  The use of other media, such as LB - Lennox may also be acceptable, but the recovery and transformation may not be as efficient.

      Helpful?

  3. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  4. Which bacterial culture medium is the best choice for my application?

    1 answer

    1. Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production.  The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.

      Helpful?

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