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RAB0748

Human TNNT1 / Troponin T, Slow Skeletal Muscle ELISA Kit

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96 wells
Usually Ships in 4 Weeks. (Orders outside of US, please allow an additional 1-2 weeks for delivery)
€ 549,00

About This Item

NACRES:
NA.32
UNSPSC Code:
41116158
Gene information:
human ... TNNT1(7138)
Input:
sample type serum
sample type plasma
sample type cell culture supernatant(s)
Species reactivity:
human
Storage temp.:
−20°C
Shipped in:
wet ice

€ 549,00

Usually Ships in 4 Weeks. (Orders outside of US, please allow an additional 1-2 weeks for delivery)
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species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable

input

sample type serum
sample type plasma
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 0.35 ng/mL
standard curve range: 0.391-25 ng/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... TNNT1(7138)

General description

Troponin T1, slow skeletal type (TNNT1) is encoded by the gene mapped to human chromosome 19q13.4. The encoded protein is composed of 261 amino acids and is expressed specifically in slow skeletal muscle.

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Troponin T plays a vital role in calcium regulation of striated muscle contraction and relaxation. The alternative splicing regulation of troponin T1, slow skeletal type (TNNT1) expression has been implicated in regulation of muscle contractility in physiological and pathophysiological adaptations. Mutation in the gene is associated with the development of Nemaline myopathy.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

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Human TNNT1 / Troponin T, Slow Skeletal Muscle ELISA Kit

Sigma-Aldrich

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Human TNNT1 / Troponin T, Slow Skeletal Muscle ELISA Kit

Anti-Troponin T antibody, Mouse monoclonal clone JLT-12, purified from hybridoma cell culture

Sigma-Aldrich

SAB4200717

Anti-Troponin T antibody, Mouse monoclonal

Human TNNI3 / Troponin I, Cardiac Muscle ELISA Kit

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Anti-Myosin Antibody, skeletal muscle heavy chain, clone 15F4 culture supernatant, clone 15F4, Chemicon&#174;

Sigma-Aldrich

MAB1549

Anti-Myosin Antibody, skeletal muscle heavy chain, clone 15F4

description

-

description

clone JLT-12, purified from hybridoma cell culture

description

-

description

culture supernatant, clone 15F4, Chemicon®

species reactivity

human

species reactivity

rat, human, mouse, bovine, rabbit, goat

species reactivity

human

species reactivity

rabbit

Gene Information

human ... TNNT1(7138)

Gene Information

human ... TNNT1(7138)

Gene Information

human ... TNNI3(7137)

Gene Information

-

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 0.35 ng/mL
standard curve range: 0.391-25 ng/mL

assay range

-

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 100 pg/mL
standard curve range: 125-8000 pg/mL

assay range

-

input

sample type serum
sample type plasma
sample type cell culture supernatant(s)

input

-

input

sample type plasma
sample type cell culture supernatant(s)
sample type serum

input

-

detection method

colorimetric

detection method

-

detection method

colorimetric

detection method

-

technique(s)

ELISA: suitable

technique(s)

immunoblotting: 0.5-1 μg/mL using rat skeletal muscle extract, immunofluorescence: suitable, immunohistochemistry: 5-10 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human skeletal muscle sections

technique(s)

ELISA: suitable

technique(s)

immunohistochemistry: suitable, western blot: suitable

Kit Components Also Available Separately

Product No.
Description
SDS & Pricing
  • RABTMB3
    ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
    SDS
  • RABSTOP3
    ELISA Stop Solution (Item I)
    SDS
  • RABWASH4
    20X Wash Buffer (Item B)
    SDS

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Codes

H290

Precautionary Statements

P234 - P390

Hazard Classifications

Met. Corr. 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

Storage Class

10 - Combustible liquids

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

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