RAB0510

Mouse VEGF ELISA Kit

for cell and tissue lysates

• Synonym(s): VEGF-A
• NACRES: NA.32
• UNSPSC Code: 41116158
• Gene information: mouse ... Vegfa(22339)
• Input: sample type cell lysate; sample type tissue lysate
• Species reactivity: mouse
• Storage temp.: −20°C
• Shipped in: wet ice

Properties

• species reactivity: mouse
• packaging: kit of 96 wells (12 strips x 8 wells)
• technique(s): ELISA: suitable, capture ELISA: suitable
• input: sample type cell lysate; sample type tissue lysate
• assay range: inter-assay cv: <12%; intra-assay cv: <10%; sensitivity: 2 pg/mL; standard curve range: 4.1-1000 pg/mL
• detection method: colorimetric
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: mouse ... Vegfa(22339)

Description

General description

The Mouse VEGF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse VEGF cell lysate and tissue lysate.

Immunogen

Recombinant Mouse VEGF-A

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Mouse VEGF ELISA Kit has been used to determine the concentration of VEGF expression in samples using ELISA.

Biochem/physiol Actions

Vascular endothelial growth factor (VEGF) is a critical factor in regulating angiogenesis in both physiological and pathological conditions. Transforming growth factor-α (TGF-α), TGF-β and platelet derived growth factor can potentially activate VEGF activity. VEGF mediates its activity through tyrosine kinase receptors, vascular endothelial growth factor receptor-1 (VEGFR-1) and VEGFR-2. VEGF produced by tumour cells and macrophages, plays a key role in cancer development by promoting endothelial cells proliferation and increasing vascular permeability. Enhanced expression of VEGF plays a key role in urologic neoplasm like bladder cancer, renal cell carcinoma, and cervical cancer. Polymorphism in VEGF gene increases the disease susceptibility. Blocking of VEGF mediated signalling pathway is a potential therapeutic strategy for cancer patients.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

• RABDIL6

  ELISA 5X Sample Diluent Buffer (Item D2)

  SDS
• RABELADE

  ELISA 5X Assay/Sample Diluent Buffer E (Item E2)

  SDS
• RABLYSIS1

  2X Cell Lysis Buffer (Item J)

  SDS
• RABSTOP3

  ELISA Stop Solution (Item I)

  SDS
• RABTMB3

  ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)

  SDS
• RABWASH4

  20X Wash Buffer (Item B)

  SDS

Safety Information

• Pictograms: GHS05
• Signal Word: Warning
• Hazard Codes: H290
• Precautionary Statements: P234 - P390
• Hazard Classifications: Met. Corr. 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• Storage Class: 12 - Non Combustible Liquids