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M9917

Mannan−Agarose

saline suspension

Synonym(s):

Mannan Binding Protein Agarose

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Size/SKUAvailabilityPrice
5 mL
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€ 436,00
25 mL
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€ 1.050,00
€ 892,50

About This Item

UNSPSC Code:
23151817
NACRES:
NA.56
MDL number:

€ 436,00


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biological source

plant

Quality Segment

form

saline suspension

technique(s)

affinity chromatography: suitable

matrix

cross-linked 4% beaded agarose

matrix activation

cyanogen bromide

matrix attachment

hydroxyl

matrix spacer

1 atom

capacity

≥30 mg/mL binding capacity (concanavalin A)

suitability

suitable for chromatography

storage temp.

2-8°C

Application

Mannan-agarose is used in protein chromatography, affinity chromatography and carbohydrate matrices. Mannan-agarose has been used in studies assessing poly(ADP-ribosyl)ation of mannose-binding lectin out of human kidney cells and to inform mannose-associated serine protease (MASP) activated components of the coagulation system. Mannan-agarose has also been used to study the innate immunity and resistance to Aeromonas salmonicida exhibited by the sea lamprey (Petromyzon marinus), a parasitic cartilaginous fish of the North American Great Lakes and a predator of many bony fish species of commercial importance to the fishing industry. Mannan-Agarose has been used to deplete rabbit anti-mannose-binding lectin (MBL) polyclonal antiserum of anti-mannan antibodies.

Physical form

Suspension in 1.0 M NaCl, 10 mM imidazole containing preservative

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PROTGA-ROPROTAA-ROAGRMP-RO
technique(s)

affinity chromatography: suitable

technique(s)

-

technique(s)

-

technique(s)

-

biological source

plant

biological source

-

biological source

-

biological source

algae (seaweed)

Quality Level

200

Quality Level

-

Quality Level

-

Quality Level

-

suitability

suitable for chromatography

suitability

-

suitability

-

suitability

-

form

saline suspension

form

suspension

form

suspension

form

powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

15-25°C, 20-25°C


Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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Questions

  1. Can you please provide details of the #m9917 mannan-agarose that you supply. E.g. the origin of the mannan immobilized (yeast?) and how it was immobilized (the data in the website referes to CNBr but I wonder how this works with a sugar. Thank you.

    1 answer
    1. The mannan used in the product of this product is from Saccharomyces cerevisiae. As stated in the 'PROPERTIES' section, the matrix attachment is at the hydroxyl group. The minimum binding capacity is 30 mg/mL using Concanavalin A.

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